Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605183848_71i6 AGGAAAGTGATCCAGAGACTGCCATATCTGGG(+CAGTGT)GATCCATCCTGTATACAGTCACCAAACCCAG NGG 10 8 17 8 24 0 100 5 10
    240605183847_5p7u AGTGTCTGGGTTTGGTGACTGTATACAGGATG(+CAGTGT)GATCCCCAGATATGGCAGTCTCTGGATCACT NGG 10 8 17 8 24 0 100 5 10
    240605183844_sipa TTCATGTATGTGTATGTATGTGTATGTCCCTG(+CAGTGT)GTACCCCTAGAGGCTGAAAAAAGGTGTCACA NGG 10 8 17 8 24 0 100 5 10
    240605183842_0umw GAGAATGTGACACCTTTTTTCAGCCTCTAGGG(+CAGTGT)GTACCAGGGACATACACATACATACACATAC NGG 10 8 17 8 24 0 100 5 10
    240605183840_7cph GGCAGTGGAATACTGCTACAGGATTGAGATGG(+CAGTGT)GATCCTGGAACAATTTGGTGGAACATAAGAA NGG 10 8 17 8 24 0 100 5 10
    240605183839_z704 TAGTGTTCTTATGTTCCACCAAATTGTTCCAG(+CAGTGT)GATCCCATCTCAATCCTGTAGCAGTATTCCA NGG 10 8 17 8 24 0 100 5 10
    240605183837_xu7y ATGCCAAATGTTTTTTTGATACATATTTGTTG(+CAGTGT)GTACCATGCCTAAAATATACAATGAAGCGAC NGG 10 8 17 8 24 0 100 5 10
    240605183836_9jkq GGAATGTCGCTTCATTGTATATTTTAGGCATG(+CAGTGT)GTACCAACAAATATGTATCAAAAAAACATTT NGG 10 8 17 8 24 0 100 5 10
    240605183835_nydk AGGAAAACAGAACACGTAAAAGTGTATTACAG(+CAGTGT)GATCCAGAAAGGAAGAAAACAACTTTGGGTC NGG 10 8 17 8 24 0 100 5 10
    240605183834_kaw1 AGCAAGACCCAAAGTTGTTTTCTTCCTTTCTG(+CAGTGT)GATCCTGTAATACACTTTTACGTGTTCTGTT NGG 10 8 17 8 24 0 100 5 10