Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605183908_f889 ATCAATGAGAGTCATTGATCTCGCACCATGTG(+CAGTGT)GATCCTGGGGATTGAATTTGGATTTATTAGA NGG 10 8 17 8 24 0 100 5 10
    240605183905_0k8j CCAAGTCTAATAAATCCAAATTCAATCCCCAG(+CAGTGT)GATCCACATGGTGCGAGATCAATGACTCTCA NGG 10 8 17 8 24 0 100 5 10
    240605183904_g6vs TCTCTCCAGTCTCCATTATCTGTTCTTTTATG(+CAGTGT)GATCCCAAAAGATTAGTCATATACAAAGGCT NGG 10 8 17 8 24 0 100 5 10
    240605183902_l3i2 GGTAAAGCCTTTGTATATGACTAATCTTTTGG(+CAGTGT)GATCCATAAAAGAACAGATAATGGAGACTGG NGG 10 8 17 8 24 0 100 5 10
    240605183900_z4r5 ATGAAATGGACAAATTTCTAGACAGATACCAG(+CAGTGT)GTACCAAAGTTAAATCAGGATCAAGTTAACG NGG 10 8 17 8 24 0 100 5 10
    240605183859_92t4 TAGATCGTTAACTTGATCCTGATTTAACTTTG(+CAGTGT)GTACCTGGTATCTGTCTAGAAATTTGTCCAT NGG 10 8 17 8 24 0 100 5 10
    240605183858_m7ph GGTGTCAGCATTTGGAGGCTGATTATGGGATG(+CAGTGT)GATCCCTGGATATGGCAGTCTCTACATGGTC NGG 10 8 17 8 24 0 100 5 10
    240605183854_ub9x GGATGGACCATGTAGAGACTGCCATATCCAGG(+CAGTGT)GATCCATCCCATAATCAGCCTCCAAATGCTG NGG 10 8 17 8 24 0 100 5 10
    240605183852_ajj0 TTCTGGATATATGCCCAGGAGAGGTATTGCGG(+CAGTGT)GATCCTCCGGTAGTACTATGTCCAATTTTCT NGG 10 8 17 8 24 0 100 5 10
    240605183850_jz28 TCCTCAGAAAATTGGACATAGTACTACCGGAG(+CAGTGT)GATCCCGCAATACCTCTCCTGGGCATATATC NGG 10 8 17 8 24 0 100 5 10