Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605184015_5ugt CAGGCTCAATACCTACCCCATGGCCCCAGGAG(+CAGTGT)GTACCCAGATATGGCTACACCCCTAGCTGAG NGG 10 8 17 8 24 0 100 5 10
    240605184013_s5hy TGGACCTCAGCTAGGGGTGTAGCCATATCTGG(+CAGTGT)GTACCTCCTGGGGCCATGGGGTAGGTATTGA NGG 10 8 17 8 24 0 100 5 10
    240605184010_2r1h CTCTTCCTCTGAGAAGGGGGAAGCTCCCCTGG(+CAGTGT)GTACCACACACACACACACACACACACACTC NGG 10 8 17 8 24 0 100 5 10
    240605184009_owx4 TGTGTGAGTGTGTGTGTGTGTGTGTGTGTGTG(+CAGTGT)GTACCCAGGGGAGCTTCCCCCTTCTCAGAGG NGG 10 8 17 8 24 0 100 5 10
    240605184007_vg9k AGCCAGATGAAACAGCCCAGTTAGGGGAATGG(+CAGTGT)GATCCACAGGCAGGCAATAGATTCAGGAACT NGG 10 8 17 8 24 0 100 5 10
    240605184005_uyrq CATTTAGTTCCTGAATCTATTGCCTGCCTGTG(+CAGTGT)GATCCCATTCCCCTAACTGGGCTGTTTCATC NGG 10 8 17 8 24 0 100 5 10
    240605184003_ngj5 GTGCAAGGATTTGGGGGGGGGGGGGCGAGGGG(+CAGTGT)GATCCTGAAACCACCACGGGGAGGAAGAGGT NGG 10 8 17 8 24 0 100 5 10
    240605184002_o421 AGGCCACCTCTTCCTCCCCGTGGTGGTTTCAG(+CAGTGT)GATCCCCCTCGCCCCCCCCCCCCCAAATCCT NGG 10 8 17 8 24 0 100 5 10
    240605183959_8jr1 CTGAGGGAGCCTGGGCCCGATGTCCTCCCATG(+CAGTGT)GATCCCTGCTTTTGTGGCCTGCTTCAGTTTC NGG 10 8 17 8 24 0 100 5 10
    240605183957_cbcd GAAGGGAAACTGAAGCAGGCCACAAAAGCAGG(+CAGTGT)GATCCATGGGAGGACATCGGGCCCAGGCTCC NGG 10 8 17 8 24 0 100 5 10