Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605183923_um7x AAAAGGAGGGTTGTTGGGGTGAGGAGAAAAGG(+CAGTGT)GTACCCCTCGAAGAAGCTCCTCAACCCCTGG NGG 10 8 17 8 24 0 100 5 10
    240605183921_wz47 ACCTTCCAGGGGTTGAGGAGCTTCTTCGAGGG(+CAGTGT)GTACCCTTTTCTCCTCACCCCAACAACCCTC NGG 10 8 17 8 24 0 100 5 10
    240605183919_omev CACAGCAGCACAAACGGACTGTGGAGACACAG(+CAGTGT)GTACCAGTTCATGAATATTCTGGGTTTCTAT NGG 10 8 17 8 24 0 100 5 10
    240605183918_3psz GAGAGATAGAAACCCAGAATATTCATGAACTG(+CAGTGT)GTACCTGTGTCTCCACAGTCCGTTTGTGCTG NGG 10 8 17 8 24 0 100 5 10
    240605183917_wbl4 CCATACCCCCAAGACTCCCCTTGGCCACAGTG(+CAGTGT)GTACCCTCCAGCTGCCCTTGGGCCCCAGCAG NGG 10 8 17 8 24 0 100 5 10
    240605183915_6gsg GCCAGCTGCTGGGGCCCAAGGGCAGCTGGAGG(+CAGTGT)GTACCACTGTGGCCAAGGGGAGTCTTGGGGG NGG 10 8 17 8 24 0 100 5 10
    240605183913_q87k TAACAGTGAACACAGCAAGGTCTGTGTTATGG(+CAGTGT)GATCCTTTTCCCAAAACAAACAAACAAACAA NGG 10 8 17 8 24 0 100 5 10
    240605183912_vesx TTTGTTTGTTTGTTTGTTTGTTTTGGGAAAAG(+CAGTGT)GATCCCATAACACAGACCTTGCTGTGTTCAC NGG 10 8 17 8 24 0 100 5 10
    240605183911_ezv8 CTGGGGAGACTGTAGGAATGATATTTCTCAAG(+CAGTGT)GTACCTCTGAAGCTGTAAGAACACTGGGCTC NGG 10 8 17 8 24 0 100 5 10
    240605183910_o3yn TCCCAGAGCCCAGTGTTCTTACAGCTTCAGAG(+CAGTGT)GTACCTTGAGAAATATCATTCCTACAGTCTC NGG 10 8 17 8 24 0 100 5 10