Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605184334_x58h TTTCTGCACTGTAAGTTATTTACAAAGTTGAG(+CAGTGT)GATCCTTTGATGGAGGAAAAAAAACCCACGT NGG 10 8 17 8 24 0 100 5 10
    240605184332_lz3d GAGAAACGTGGGTTTTTTTTCCTCCATCAAAG(+CAGTGT)GATCCTCAACTTTGTAAATAACTTACAGTGC NGG 10 8 17 8 24 0 100 5 10
    240605184331_a1qs GGTGGCACTTCCTGCCAGGACCTCCCTAATGG(+CAGTGT)GTACCTGGCAGAAGAGGTAGGTTACCTGGGT NGG 10 8 17 8 24 0 100 5 10
    240605184329_wc47 AACTGACCCAGGTAACCTACCTCTTCTGCCAG(+CAGTGT)GTACCCATTAGGGAGGTCCTGGCAGGAAGTG NGG 10 8 17 8 24 0 100 5 10
    240605184328_ojnr TCCTTGAGAAACACATTTGGGAAGTTTCTTCG(+CAGTGT)GATCCTTCTTTCTGAGCTCAAGGAAGAAAAT NGG 10 8 17 8 24 0 100 5 10
    240605184327_gd70 GTCTGATTTTCTTCCTTGAGCTCAGAAAGAAG(+CAGTGT)GATCCGAAGAAACTTCCCAAATGTGTTTCTC NGG 10 8 17 8 24 0 100 5 10
    240605184325_x447 CCCCGAGAACTCAGGAGGCCTAAGTCCGTGGG(+CAGTGT)GATCCTCATTGCCATTTTGAAGACATGTACT NGG 10 8 17 8 24 0 100 5 10
    240605184323_4gyz ATCCCAGTACATGTCTTCAAAATGGCAATGAG(+CAGTGT)GATCCCCACGGACTTAGGCCTCCTGAGTTCT NGG 10 8 17 8 24 0 100 5 10
    240605184322_sgvq GAGGGGTGGTGTGTGCACAGGGCCATTTCACG(+CAGTGT)GTACCCAATTTCAGAGGACCACAGGGTGAAA NGG 10 8 17 8 24 0 100 5 10
    240605184320_t76g CAGTATTTCACCCTGTGGTCCTCTGAAATTGG(+CAGTGT)GTACCGTGAAATGGCCCTGTGCACACACCAC NGG 10 8 17 8 24 0 100 5 10