Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605184746_w5q1 GGGAAACAGCACAGACAATGAATGGTAACAGG(+CAGTGT)GTACCCAGCAGGGGAGGGATGGCAGAGCCCC NGG 10 8 17 8 24 0 100 5 10
    240605184744_39fk ACACTGCCTGTGCCCCAACTCTGCCTCACTCG(+CAGTGT)GATCCGTCTGCCCTGTGGGGACATGAGTGTC NGG 10 8 17 8 24 0 100 5 10
    240605184743_t22a ACAGAGACACTCATGTCCCCACAGGGCAGACG(+CAGTGT)GATCCGAGTGAGGCAGAGTTGGGGCACAGGC NGG 10 8 17 8 24 0 100 5 10
    240605184742_10j4 TTTTCTTGGAAGAAAGGACTCTAGGGTGGATG(+CAGTGT)GTACCAGGCCTTCAGGGTTAGATCATCTGTC NGG 10 8 17 8 24 0 100 5 10
    240605184740_jhll CACTTGACAGATGATCTAACCCTGAAGGCCTG(+CAGTGT)GTACCATCCACCCTAGAGTCCTTTCTTCCAA NGG 10 8 17 8 24 0 100 5 10
    240605184739_iw92 CTAGAGAGCACCAGCTCCAGGTCCATGACTGG(+CAGTGT)GTACCACTGTTTGCCCTGGCCTGTCTCCTGA NGG 10 8 17 8 24 0 100 5 10
    240605184737_3zyr GCTCCTCAGGAGACAGGCCAGGGCAAACAGTG(+CAGTGT)GTACCCAGTCATGGACCTGGAGCTGGTGCTC NGG 10 8 17 8 24 0 100 5 10
    240605184736_957w TGTCACCAGGGGGTGTGCAAGCCGGCATAAGG(+CAGTGT)GATCCCTTGGAGATACTCAAGAAGAGGGGCT NGG 10 8 17 8 24 0 100 5 10
    240605184733_2kh2 CAGGGAGCCCCTCTTCTTGAGTATCTCCAAGG(+CAGTGT)GATCCCTTATGCCGGCTTGCACACCCCCTGG NGG 10 8 17 8 24 0 100 5 10
    240605184732_si4i AGTCTTCAGCAACCACCATGGCCTGATATTGG(+CAGTGT)GTACCACCCTGCAGGTTATCAGCTACACTCC NGG 10 8 17 8 24 0 100 5 10