Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605184835_sk2a TAATCTCAGCCTTTGGAAGGCAGAGACAGGAG(+CAGTGT)GATCCCTGGTGCTCGCATGCCAGTCAGTTTA NGG 10 8 17 8 24 0 100 5 10
    240605184834_slvk CCCCCACAGCCCTTTGATGTTACCAGGCACCG(+CAGTGT)GATCCCCATGGCTTCCGTCACCCCTGGTCCT NGG 10 8 17 8 24 0 100 5 10
    240605184832_e0wx TGGTAAGGACCAGGGGTGACGGAAGCCATGGG(+CAGTGT)GATCCGGTGCCTGGTAACATCAAAGGGCTGT NGG 10 8 17 8 24 0 100 5 10
    240605184831_haqs TATTTTTCCCTTTCAAATAAAACACCAGTCAG(+CAGTGT)GTACCTTTATCCCAGTCTTAACTGTCCCAAA NGG 10 8 17 8 24 0 100 5 10
    240605184829_j8mn CCAGATTTGGGACAGTTAAGACTGGGATAAAG(+CAGTGT)GTACCTGACTGGTGTTTTATTTGAAAGGGAA NGG 10 8 17 8 24 0 100 5 10
    240605184827_d0ne ACACCCACACTATATAAAGTACTTAGTAGGGG(+CAGTGT)GTACCCCGTAGTGTGATTTAAGTCATTGGTG NGG 10 8 17 8 24 0 100 5 10
    240605184825_qmww AATCTCACCAATGACTTAAATCACACTACGGG(+CAGTGT)GTACCCCCTACTAAGTACTTTATATAGTGTG NGG 10 8 17 8 24 0 100 5 10
    240605184824_5tew TGGCAAACCACTGTCTGCTATGGTTCTGAGAG(+CAGTGT)GATCCAGAGCCTTGGTATTCAAAAGCCAAGT NGG 10 8 17 8 24 0 100 5 10
    240605184823_aqm8 CCGACACTTGGCTTTTGAATACCAAGGCTCTG(+CAGTGT)GATCCTCTCAGAACCATAGCAGACAGTGGTT NGG 10 8 17 8 24 0 100 5 10
    240605184821_t03o TGAAGTCCATTGCAGCTACAGTTGCTGATGGG(+CAGTGT)GATCCCATAGGAGAAAATGGACCAGGGAAGA NGG 10 8 17 8 24 0 100 5 10