Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605183710_pabg GAAAGGCCTGGAAGAAATGGAGATGCTGTGTG(+CAGTGT)GTACCTATCAACCGAGCAGTAAGTAAGTGAC NGG 10 8 17 8 24 0 100 5 10
    240605183709_1cpe GCCACAGCTCAGGTCTTCTCCACACTCGTCAG(+CAGTGT)GTACCTGTGCTATGGAGTTTCTGCCCGAGTC NGG 10 8 17 8 24 0 100 5 10
    240605183707_swfk TTGTTGACTCGGGCAGAAACTCCATAGCACAG(+CAGTGT)GTACCTGACGAGTGTGGAGAAGACCTGAGCT NGG 10 8 17 8 24 0 100 5 10
    240605183706_blub CATGGATTTGGGGATATTTTCCAGCTAGAATG(+CAGTGT)GATCCAGAATTGAATGGTGCTTAAATTGAGA NGG 10 8 17 8 24 0 100 5 10
    240605183705_i1zo TTATTTCTCAATTTAAGCACCATTCAATTCTG(+CAGTGT)GATCCATTCTAGCTGGAAAATATCCCCAAAT NGG 10 8 17 8 24 0 100 5 10
    240605183703_5vmm GGTATTTTCAGCTGCAGTTCTCCTCCTCTCTG(+CAGTGT)GATCCTGGCTTGGGGTGCTTTTCCAGCATCC NGG 10 8 17 8 24 0 100 5 10
    240605183702_95u4 ACTGTGGATGCTGGAAAAGCACCCCAAGCCAG(+CAGTGT)GATCCAGAGAGGAGGAGAACTGCAGCTGAAA NGG 10 8 17 8 24 0 100 5 10
    240605183700_z509 CTATTAGGAGAGTAGATGTTAATGTGTTTTGG(+CAGTGT)GTACCCATCTTTGCAACATGTCTGATCCACG NGG 10 8 17 8 24 0 100 5 10
    240605183658_wh4v TGGCACGTGGATCAGACATGTTGCAAAGATGG(+CAGTGT)GTACCCAAAACACATTAACATCTACTCTCCT NGG 10 8 17 8 24 0 100 5 10
    240605183657_aziq CTATTAGGAGAGTAGATGTTAATGTGTTTTGG(+CAGTGT)GTACCCATCTTTGCAACATGTCTGATCCACG NGG 10 8 17 8 24 0 100 5 10