Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605183633_5nh9 GTCACCAGTCACTGGGTCTAGGAGATGTCTTG(+CAGTGT)GTACCCGTCTGTCTGACATTGACAATGACCT NGG 10 8 17 8 24 0 100 5 10
    240605183631_540z CTTTCAGGTCATTGTCAATGTCAGACAGACGG(+CAGTGT)GTACCAAGACATCTCCTAGACCCAGTGACTG NGG 10 8 17 8 24 0 100 5 10
    240605183629_1ot2 GCAAACAGCAAGAGGCTTTATTGGGAACACGG(+CAGTGT)GTACCCGGGCGACGCAGTCTATCGGAGGACT NGG 10 8 17 8 24 0 100 5 10
    240605183627_3zts GCGCCAGTCCTCCGATAGACTGCGTCGCCCGG(+CAGTGT)GTACCCGTGTTCCCAATAAAGCCTCTTGCTG NGG 10 8 17 8 24 0 100 5 10
    240605183625_jiog TGACCACGGCACCTTCTGACCCGCTCATCCTG(+CAGTGT)GATCCTGTGGGTTTGGGGTGCGTGGGAACGC NGG 10 8 17 8 24 0 100 5 10
    240605183622_je15 GAAGTGCGTTCCCACGCACCCCAAACCCACAG(+CAGTGT)GATCCAGGATGAGCGGGTCAGAAGGTGCCGT NGG 10 8 17 8 24 0 100 5 10
    240605183620_a9xg AGAAATGGTACAATGTCAGATATCTCAGGGAG(+CAGTGT)GTACCCTGTGGATCTGTATTCCAACGGGAGT NGG 10 8 17 8 24 0 100 5 10
    240605183618_w212 TCAGGACTCCCGTTGGAATACAGATCCACAGG(+CAGTGT)GTACCTCCCTGAGATATCTGACATTGTACCA NGG 10 8 17 8 24 0 100 5 10
    240605183617_5b4p AGGGCTTGTTGAGTAGGCATCTGGAATGACCG(+CAGTGT)GATCCGGGCATCAGGCCGCTGATGGGCATAG NGG 10 8 17 8 24 0 100 5 10
    240605183615_l2n9 CCACTCTATGCCCATCAGCGGCCTGATGCCCG(+CAGTGT)GATCCGGTCATTCCAGATGCCTACTCAACAA NGG 10 8 17 8 24 0 100 5 10