Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605183110_8rkv CTTTTCAGATAGCCCTGGCTGGTGGGGGAATG(+CAGTGT)GATCCCTGAGAACACGGGCATGTCACACAGG NGG 10 8 17 8 24 0 100 5 10
    240605183108_9jfa CATTCCCTGTGTGACATGCCCGTGTTCTCAGG(+CAGTGT)GATCCATTCCCCCACCAGCCAGGGCTATCTG NGG 10 8 17 8 24 0 100 5 10
    240605183107_5t22 GCAGGGGGAGAGAGGAGCTGAGGAAATGTGGG(+CAGTGT)GATCCTGGGGGCTCGGACCGGGGTGAGTGTC NGG 10 8 17 8 24 0 100 5 10
    240605183104_e87f ACATAGACACTCACCCCGGTCCGAGCCCCCAG(+CAGTGT)GATCCCCACATTTCCTCAGCTCCTCTCTCCC NGG 10 8 17 8 24 0 100 5 10
    240605183102_sn08 CTATAGATGGAGATCTGAAGATTAGACTCTAG(+CAGTGT)GATCCACAGATGAGAGAGTATACGTGACATT NGG 10 8 17 8 24 0 100 5 10
    240605183101_ogrp AGGCAAATGTCACGTATACTCTCTCATCTGTG(+CAGTGT)GATCCTAGAGTCTAATCTTCAGATCTCCATC NGG 10 8 17 8 24 0 100 5 10
    240605183100_wh3b CCTCACCAGGTAGCTCTCTAGCCCCAAGAAAG(+CAGTGT)GATCCCACCAGAAACAGTATCAGATGTCATA NGG 10 8 17 8 24 0 100 5 10
    240605183058_5d09 TAATTTATGACATCTGATACTGTTTCTGGTGG(+CAGTGT)GATCCTTTCTTGGGGCTAGAGAGCTACCTGG NGG 10 8 17 8 24 0 100 5 10
    240605183056_adzj CAAGCTGCTGAAACCTTTAAAATGTATCAAGG(+CAGTGT)GATCCATAGCCTCTACCATCCGGAATGCTAA NGG 10 8 17 8 24 0 100 5 10
    240605183055_2lf8 ATCCTTTAGCATTCCGGATGGTAGAGGCTATG(+CAGTGT)GATCCCTTGATACATTTTAAAGGTTTCAGCA NGG 10 8 17 8 24 0 100 5 10