Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605183213_mn12 CCTGCAGCCACCTAAGAGTGTAGGTGTTCCAG(+CAGTGT)GATCCTCCTCTGGGGATATCTTAGAGAAAGA NGG 10 8 17 8 24 0 100 5 10
    240605183211_0vgd GGGTCTCTTTCTCTAAGATATCCCCAGAGGAG(+CAGTGT)GATCCTGGAACACCTACACTCTTAGGTGGCT NGG 10 8 17 8 24 0 100 5 10
    240605183210_pm61 CCTGCAGCCACCTAAGAGTGTAGGTGTTCCAG(+CAGTGT)GATCCTCCTCTGGGGATATCTTAGAGAAAGA NGG 10 8 17 8 24 0 100 5 10
    240605183208_spxt GGGTCTCTTTCTCTAAGATATCCCCAGAGGAG(+CAGTGT)GATCCTGGAACACCTACACTCTTAGGTGGCT NGG 10 8 17 8 24 0 100 5 10
    240605183206_ogsg CCTGCAGCCACCTAAGAGTGTAGGTGTTCCAG(+CAGTGT)GATCCTCCTCTGGGGATATCTTAGAGAAAGA NGG 10 8 17 8 24 0 100 5 10
    240605183204_xodq GGGTCTCTTTCTCTAAGATATCCCCAGAGGAG(+CAGTGT)GATCCTGGAACACCTACACTCTTAGGTGGCT NGG 10 8 17 8 24 0 100 5 10
    240605183203_kmsw CATGTCTCTGCAGAGGCCAGAGGAGGGTATTG(+CAGTGT)GATCCCTAGAAGCTGGAGTTAGAATAGGTAA NGG 10 8 17 8 24 0 100 5 10
    240605183201_1i6d GAGACTTACCTATTCTAACTCCAGCTTCTAGG(+CAGTGT)GATCCAATACCCTCCTCTGGCCTCTGCAGAG NGG 10 8 17 8 24 0 100 5 10
    240605183200_qrbr GTTCCAGCAAACAGGAAATGGGGATGCTGAAG(+CAGTGT)GATCCCAGGGAGGGGACGTTACAACCACAGC NGG 10 8 17 8 24 0 100 5 10
    240605183158_9sbs AATCAGCTGTGGTTGTAACGTCCCCTCCCTGG(+CAGTGT)GATCCTTCAGCATCCCCATTTCCTGTTTGCT NGG 10 8 17 8 24 0 100 5 10