Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605183259_r1qb GAGTGGCACAAGTCTGTAATTGCAGTGTTTGG(+CAGTGT)GATCCTAAGGCAAGATGATCTTGAGTTCCAG NGG 10 8 17 8 24 0 100 5 10
    240605183257_le8c GGATCCCTCCTGGGCTTGTCAGAGAGTTTTGG(+CAGTGT)GTACCAAAGAGGTCTAGTAGACTCCCCAGAA NGG 10 8 17 8 24 0 100 5 10
    240605183256_kat5 GCCACTTCTGGGGAGTCTACTAGACCTCTTTG(+CAGTGT)GTACCCAAAACTCTCTGACAAGCCCAGGAGG NGG 10 8 17 8 24 0 100 5 10
    240605183255_9k38 CACCTGCAACCTTCCACGGTGCATGCTCTGTG(+CAGTGT)GATCCCTCCTGGGCTTGTCAGAGAGTTTTGG NGG 10 8 17 8 24 0 100 5 10
    240605183253_wnte GGTACCCAAAACTCTCTGACAAGCCCAGGAGG(+CAGTGT)GATCCACAGAGCATGCACCGTGGAAGGTTGC NGG 10 8 17 8 24 0 100 5 10
    240605183251_pvz8 CCCATTACGCCTGGTACAAGTCTGTCCTCAGG(+CAGTGT)GATCCAGTGTGAGAAATACAGATGGATGGCC NGG 10 8 17 8 24 0 100 5 10
    240605183250_8qh2 TGCGAGGCCATCCATCTGTATTTCTCACACTG(+CAGTGT)GATCCCTGAGGACAGACTTGTACCAGGCGTA NGG 10 8 17 8 24 0 100 5 10
    240605183248_6dq1 GGGGGGAACGGCAGCTGAGCAGGTGTAGGGTG(+CAGTGT)GATCCTGCTGGTGACCAGGGCAGTCATCCAT NGG 10 8 17 8 24 0 100 5 10
    240605183247_yo6g GCTTCATGGATGACTGCCCTGGTCACCAGCAG(+CAGTGT)GATCCACCCTACACCTGCTCAGCTGCCGTTC NGG 10 8 17 8 24 0 100 5 10
    240605183246_ol56 CAGTCCAGTCAAGTGAGAAATGGAAAGAGTTG(+CAGTGT)GTACCACGGGGCCAGGGAAACAAAGTGAGAT NGG 10 8 17 8 24 0 100 5 10