Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605183227_gkr6 ATAAATCACCTCAAGATGCCAGGACCCAAGTG(+CAGTGT)GATCCACGTTGAGGTGCATGGACTCTGGTGG NGG 10 8 17 8 24 0 100 5 10
    240605183224_b7tv TTCTGCTTCTTACCCTCCCTTCCAAGCCCTGG(+CAGTGT)GATCCTAAAAGTCCCGCCTTTGTCTGTCCTG NGG 10 8 17 8 24 0 100 5 10
    240605183223_z92n CTAGGCAGGACAGACAAAGGCGGGACTTTTAG(+CAGTGT)GATCCCAGGGCTTGGAAGGGAGGGTAAGAAG NGG 10 8 17 8 24 0 100 5 10
    240605183222_rk2s GATTCTCTGCTCTTTGTATGACCGGGTGAAAG(+CAGTGT)GTACCTGTCGTCTTTACAAGTTAGTTTGGAC NGG 10 8 17 8 24 0 100 5 10
    240605183220_08n0 ATACAGTCCAAACTAACTTGTAAAGACGACAG(+CAGTGT)GTACCTTTCACCCGGTCATACAAAGAGCAGA NGG 10 8 17 8 24 0 100 5 10
    240605183219_lowo TATAAGTTTAGTATACAATGGATCAAATTAAG(+CAGTGT)GATCCTGCTCTCTTTCCTTTGGAGGTAGTTC NGG 10 8 17 8 24 0 100 5 10
    240605183218_wh3g ACGTCGAACTACCTCCAAAGGAAAGAGAGCAG(+CAGTGT)GATCCTTAATTTGATCCATTGTATACTAAAC NGG 10 8 17 8 24 0 100 5 10
    240605183216_vd05 CCTGCAGCCACCTAAGAGTGTAGGTGTTCCAG(+CAGTGT)GATCCTCCTCTGGGGATATCTTAGAGAAAGA NGG 10 8 17 8 24 0 100 5 10
    240605183215_87r5 GGGTCTCTTTCTCTAAGATATCCCCAGAGGAG(+CAGTGT)GATCCTGGAACACCTACACTCTTAGGTGGCT NGG 10 8 17 8 24 0 100 5 10
    240605183213_mn12 CCTGCAGCCACCTAAGAGTGTAGGTGTTCCAG(+CAGTGT)GATCCTCCTCTGGGGATATCTTAGAGAAAGA NGG 10 8 17 8 24 0 100 5 10