Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605183338_oaad GCTGTCTTCAGAGATACCAGAAGATGGCATTG(+CAGTGT)GATCCCATTACAGATGGTTATGAGCCACTAT NGG 10 8 17 8 24 0 100 5 10
    240605183337_24qe AGTCACCGCCTGGCAAGGGCCATGGTAGTTAG(+CAGTGT)GTACCGAAATGCTCCTGTACAGATGCAAACC NGG 10 8 17 8 24 0 100 5 10
    240605183336_blyp GTACAGGTTTGCATCTGTACAGGAGCATTTCG(+CAGTGT)GTACCTAACTACCATGGCCCTTGCCAGGCGG NGG 10 8 17 8 24 0 100 5 10
    240605183333_k3r3 CTAGCTGGGAGTGCCGGCAGAGGCAGGGCTGG(+CAGTGT)GTACCACAGGGCCAGCTTGAGTGTGGGAGGA NGG 10 8 17 8 24 0 100 5 10
    240605183332_nsas CAAGTTCCTCCCACACTCAAGCTGGCCCTGTG(+CAGTGT)GTACCCAGCCCTGCCTCTGCCGGCACTCCCA NGG 10 8 17 8 24 0 100 5 10
    240605183329_5ud3 TCCATGCCTCCCCACCCTTGTTCACGCCCATG(+CAGTGT)GATCCCCTGGCCTTGATGTCTGCCCCTCTTT NGG 10 8 17 8 24 0 100 5 10
    240605183326_2cxs CTGAGAAAGAGGGGCAGACATCAAGGCCAGGG(+CAGTGT)GATCCATGGGCGTGAACAAGGGTGGGGAGGC NGG 10 8 17 8 24 0 100 5 10
    240605183325_uy6z CTCCCCAGAGGACGCAGTGAGTCCCCAGCTGG(+CAGTGT)GATCCCACCACACTGGACAGGAGCCAGGAGT NGG 10 8 17 8 24 0 100 5 10
    240605183323_d5gt AGAGCACTCCTGGCTCCTGTCCAGTGTGGTGG(+CAGTGT)GATCCCAGCTGGGGACTCACTGCGTCCTCTG NGG 10 8 17 8 24 0 100 5 10
    240605183321_fy2t TGACTCCTTCAGTGTCTATAGAAGAAAAGGTG(+CAGTGT)GATCCTGGAGCCTACAGAAAACTCAACCCTT NGG 10 8 17 8 24 0 100 5 10