Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605183414_f1m0 CCCCTTGCTGTAAATGCTTGTGAGTGTATATG(+CAGTGT)GATCCCGAATAAATGAGTGTAGTCCCAGCCA NGG 10 8 17 8 24 0 100 5 10
    240605183412_kij3 CCATTATAACCCTGTTTCCACACGTCTGCCTG(+CAGTGT)GTACCATGCTCACCATTAACAAATTATGCCG NGG 10 8 17 8 24 0 100 5 10
    240605183410_zi6q TCTTTCGGCATAATTTGTTAATGGTGAGCATG(+CAGTGT)GTACCAGGCAGACGTGTGGAAACAGGGTTAT NGG 10 8 17 8 24 0 100 5 10
    240605183409_ipcn ACCCAGCCCCCCATTTGATATGTAGGCAGGAG(+CAGTGT)GTACCTAAGTAGGAAACAAGTCAGATTATAT NGG 10 8 17 8 24 0 100 5 10
    240605183407_abpl TAAGCATATAATCTGACTTGTTTCCTACTTAG(+CAGTGT)GTACCTCCTGCCTACATATCAAATGGGGGGC NGG 10 8 17 8 24 0 100 5 10
    240605183404_7j6z TTTTCCTCTGCAGCGCCGCTCTCTGCAGCCGG(+CAGTGT)GATCCACCACCCACTTTCTCACGGCCATCTC NGG 10 8 17 8 24 0 100 5 10
    240605183402_xbzn TGGAAGAGATGGCCGTGAGAAAGTGGGTGGTG(+CAGTGT)GATCCCGGCTGCAGAGAGCGGCGCTGCAGAG NGG 10 8 17 8 24 0 100 5 10
    240605183400_lmfv TGGGAGGAAGAGGAAGGCAGCTCCTGCTCCTG(+CAGTGT)GATCCCCCTTGAAGATCCATTCTCCATAGAA NGG 10 8 17 8 24 0 100 5 10
    240605183358_75gc TTTGCTTCTATGGAGAATGGATCTTCAAGGGG(+CAGTGT)GATCCAGGAGCAGGAGCTGCCTTCCTCTTCC NGG 10 8 17 8 24 0 100 5 10
    240605183356_wkg3 CAGACTGGGAGGGGACGGGTAAGAGAGGTGGG(+CAGTGT)GTACCAGACTCCAACAGGCTCCAAAGAAATA NGG 10 8 17 8 24 0 100 5 10