Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605183430_n14y TCTTAGACAAGTGGGTCATCCTGTGGCTAAAG(+CAGTGT)GATCCAAATGTTTGGACAGCTATGTCACAAG NGG 10 8 17 8 24 0 100 5 10
    240605183429_36xf TATTTCTCTCATTTGAAGATTTTCTCAACAGG(+CAGTGT)GTACCTCCTCTGAACAACAAGCTTCCCACAT NGG 10 8 17 8 24 0 100 5 10
    240605183427_28xf TGAGAATGTGGGAAGCTTGTTGTTCAGAGGAG(+CAGTGT)GTACCCTGTTGAGAAAATCTTCAAATGAGAG NGG 10 8 17 8 24 0 100 5 10
    240605183426_16nb TGAAATGGCCTCAGCAAGAATAAAAGATGAAG(+CAGTGT)GATCCTGTGGACTTTCTTCTCCTGCTACAAA NGG 10 8 17 8 24 0 100 5 10
    240605183424_utsu ATACATTTGTAGCAGGAGAAGAAAGTCCACAG(+CAGTGT)GATCCTTCATCTTTTATTCTTGCTGAGGCCA NGG 10 8 17 8 24 0 100 5 10
    240605183423_v5iy GCTGTCATCAGACACATCAGAAGAGGGCATCG(+CAGTGT)GATCCCATTACAGATGCTTGTGAGCCACCAT NGG 10 8 17 8 24 0 100 5 10
    240605183421_my4u ACCACATGGTGGCTCACAAGCATCTGTAATGG(+CAGTGT)GATCCGATGCCCTCTTCTGATGTGTCTGATG NGG 10 8 17 8 24 0 100 5 10
    240605183419_nc6b CCATTATGACCCCGTTTCCACACATCCGCCTG(+CAGTGT)GTACCGTGCTCACCATGAACTATGCAGAAAG NGG 10 8 17 8 24 0 100 5 10
    240605183417_hlem GCTTTCTTTCTGCATAGTTCATGGTGAGCACG(+CAGTGT)GTACCAGGCGGATGTGTGGAAACGGGGTCAT NGG 10 8 17 8 24 0 100 5 10
    240605183415_ba7y CCAGTTGGCTGGGACTACACTCATTTATTCGG(+CAGTGT)GATCCATATACACTCACAAGCATTTACAGCA NGG 10 8 17 8 24 0 100 5 10