Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605183500_9w28 CTCCCTCAAACATCTTAGGTACCCTAGGAACG(+CAGTGT)GATCCACCCAAACACTGAATTTGCAAACTAG NGG 10 8 17 8 24 0 100 5 10
    240605183459_mjrl CTGGAGATTGACCTGTGTAAAGTAACGTTTAG(+CAGTGT)GATCCTGACGTGACTGTCATCTCCAAGACTT NGG 10 8 17 8 24 0 100 5 10
    240605183458_6sj9 GGAGGAAGTCTTGGAGATGACAGTCACGTCAG(+CAGTGT)GATCCTAAACGTTACTTTACACAGGTCAATC NGG 10 8 17 8 24 0 100 5 10
    240605183457_dzmy GTGACTCTGGGGTTCAAGGCAGAGACTATGTG(+CAGTGT)GATCCCAGGGTCATACCGCCAAGCCAGTCTA NGG 10 8 17 8 24 0 100 5 10
    240605183454_8zc4 TCAGCTAGACTGGCTTGGCGGTATGACCCTGG(+CAGTGT)GATCCACATAGTCTCTGCCTTGAACCCCAGA NGG 10 8 17 8 24 0 100 5 10
    240605183453_vku0 TCAGCACCCAAGATTGGGGGATAACTAGACTG(+CAGTGT)GTACCAGTTCATATATGCCTTATAAAATCTG NGG 10 8 17 8 24 0 100 5 10
    240605183452_jvci TCAAACAGATTTTATAAGGCATATATGAACTG(+CAGTGT)GTACCAGTCTAGTTATCCCCCAATCTTGGGT NGG 10 8 17 8 24 0 100 5 10
    240605183450_0s4n TCTGTACCCACTGTCTGTCTGCTCCCCCGATG(+CAGTGT)GTACCTAAGGCAGCCTGCTGAATATACCCAC NGG 10 8 17 8 24 0 100 5 10
    240605183448_wvd1 AGGTTGTGGGTATATTCAGCAGGCTGCCTTAG(+CAGTGT)GTACCATCGGGGGAGCAGACAGACAGTGGGT NGG 10 8 17 8 24 0 100 5 10
    240605183446_x1za CCAGGAGGCCTGACCTGGCCGGAGTGGACAGG(+CAGTGT)GATCCAGAAACGAAGCCAAATAAGGGAAGAG NGG 10 8 17 8 24 0 100 5 10