Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605174743_r07e TACTTCAGCTAGTGTGCAAGCCGGCACCGAGG(+CAGTGT)GTACCACCTTCTCTCAAGCGACATCACTTTT NGG 10 8 17 8 24 0 100 5 10
    240605174742_jyda TTGGGGTCCCTCAGCACTTTGCCCTGATTTCG(+CAGTGT)GATCCTGAGTAACAGATACCACTGAATTGCT NGG 10 8 17 8 24 0 100 5 10
    240605174741_jur2 GTTACAGCAATTCAGTGGTATCTGTTACTCAG(+CAGTGT)GATCCGAAATCAGGGCAAAGTGCTGAGGGAC NGG 10 8 17 8 24 0 100 5 10
    240605174738_rpz5 GCGGCCCCAGCAACCGGGACGGAGGCTGCCGG(+CAGTGT)GATCCAGCCCGCGCCGCCAACGTGCGGTCGG NGG 10 8 17 8 24 0 100 5 10
    240605174737_dozo GGGTTCCGACCGCACGTTGGCGGCGCGGGCTG(+CAGTGT)GATCCCGGCAGCCTCCGTCCCGGTTGCTGGG NGG 10 8 17 8 24 0 100 5 10
    240605174735_fjan ATGTTCCTGTATTTCTGCCCCTTGAGTGTTGG(+CAGTGT)GATCCCAGGCACAGACCTCACCACCCAGCCA NGG 10 8 17 8 24 0 100 5 10
    240605174733_1ske TCCTCTGGCTGGGTGGTGAGGTCTGTGCCTGG(+CAGTGT)GATCCCAACACTCAAGGGGCAGAAATACAGG NGG 10 8 17 8 24 0 100 5 10
    240605174731_lfom AAGGAACCCACTATAATTAAGAGTAGCAAGAG(+CAGTGT)GTACCAGTAGGGGCTGTGTAATTCAATAAAA NGG 10 8 17 8 24 0 100 5 10
    240605174730_n8y6 TCTTGTTTTATTGAATTACACAGCCCCTACTG(+CAGTGT)GTACCTCTTGCTACTCTTAATTATAGTGGGT NGG 10 8 17 8 24 0 100 5 10
    240605174728_bd9q GGAGATCTAGTGTACTCTTCTGACCTCCAAAG(+CAGTGT)GTACCAGGTGAACATCCGGATGAATGCAGGC NGG 10 8 17 8 24 0 100 5 10