Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605174655_m8se GAACCAGGAAGAATGTCCCACCGTTCAGGTTG(+CAGTGT)GTACCAGACTCAGAAAGCATGGCTCTCTGGC NGG 10 8 17 8 24 0 100 5 10
    240605174653_agje TTAAAATAGAGAAATTGACAGCATTCACCATG(+CAGTGT)GTACCCAGGGGTTAGAGCTGGTGTCAAGAGT NGG 10 8 17 8 24 0 100 5 10
    240605174650_6x1l CGATTACTCTTGACACCAGCTCTAACCCCTGG(+CAGTGT)GTACCATGGTGAATGCTGTCAATTTCTCTAT NGG 10 8 17 8 24 0 100 5 10
    240605174649_j35f CTCCTCCTCCCTCCTCCCTCAGAAGTTCTGAG(+CAGTGT)GATCCTCGACAAGGTCACTGTCCACGACACT NGG 10 8 17 8 24 0 100 5 10
    240605174647_y9mn TCATTAGTGTCGTGGACAGTGACCTTGTCGAG(+CAGTGT)GATCCTCAGAACTTCTGAGGGAGGAGGGAGG NGG 10 8 17 8 24 0 100 5 10
    240605174646_m15o GGTTGGGAATGTGGCAAATCATGGCCAGTCTG(+CAGTGT)GATCCCCTGGTCAGTGCTCTCTGTATCTGGG NGG 10 8 17 8 24 0 100 5 10
    240605174644_zsb6 AGGAGCCCAGATACAGAGAGCACTGACCAGGG(+CAGTGT)GATCCAGACTGGCCATGATTTGCCACATTCC NGG 10 8 17 8 24 0 100 5 10
    240605174642_odzu TTAATTAGTCTAGCTACCCAGCTTGCTGTAGG(+CAGTGT)GATCCCTTTGTGCTTTTTTAAGGAGTAGAAA NGG 10 8 17 8 24 0 100 5 10
    240605174640_ahho TGAATTTTCTACTCCTTAAAAAAGCACAAAGG(+CAGTGT)GATCCCTACAGCAAGCTGGGTAGCTAGACTA NGG 10 8 17 8 24 0 100 5 10
    240605174639_c1hu ACATCTGGGGTCAAGAGAAAGGGGAGTGAGAG(+CAGTGT)GATCCTCAAAGGATATCTCCTTTGCCCTTCC NGG 10 8 17 8 24 0 100 5 10