Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605174622_kqk8 TGAGATTTGCATAAAAGGAGTGCTTTATTAAG(+CAGTGT)GATCCAAATCTCAAAGCCTCCTGTGAGTTGT NGG 10 8 17 8 24 0 100 5 10
    240605174621_3afl TTTTTCTTTAAAAAGCTTAAAGATTGGCATGG(+CAGTGT)GTACCGCTCTACTAATTATCTAAAACACAAC NGG 10 8 17 8 24 0 100 5 10
    240605174619_5zmk AAGCAGTTGTGTTTTAGATAATTAGTAGAGCG(+CAGTGT)GTACCCATGCCAATCTTTAAGCTTTTTAAAG NGG 10 8 17 8 24 0 100 5 10
    240605174618_03tq AACAAAGACACAGGAATAACTATAATGTGTAG(+CAGTGT)GATCCAGTGCATCTTGGCAACTGAAGAGCCT NGG 10 8 17 8 24 0 100 5 10
    240605174617_uo2d AGAAAAGGCTCTTCAGTTGCCAAGATGCACTG(+CAGTGT)GATCCTACACATTATAGTTATTCCTGTGTCT NGG 10 8 17 8 24 0 100 5 10
    240605174615_qidf TCTCTAATGACAAAAGCATCCTGCCACACCTG(+CAGTGT)GTACCACAGGACCTGGAGGCTAAGGCAGAGA NGG 10 8 17 8 24 0 100 5 10
    240605174613_fa4m GAAAGTCTCTGCCTTAGCCTCCAGGTCCTGTG(+CAGTGT)GTACCAGGTGTGGCAGGATGCTTTTGTCATT NGG 10 8 17 8 24 0 100 5 10
    240605174611_np7u CTGAGCAGTGTATATTCTTAAACAGTGCCAAG(+CAGTGT)GATCCTGTCTTCAAACAGTTTTATCAACTGA NGG 10 8 17 8 24 0 100 5 10
    240605174610_c0aw ATGTTTCAGTTGATAAAACTGTTTGAAGACAG(+CAGTGT)GATCCTTGGCACTGTTTAAGAATATACACTG NGG 10 8 17 8 24 0 100 5 10
    240605174609_9nli GTTTAAAAAAATACAATTAACATGGGAACAAG(+CAGTGT)GATCCCATTTTTTTAAAAAGAAAAAATATAT NGG 10 8 17 8 24 0 100 5 10