Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605174550_da81 GCTTCACCCTGTGAGGAAGCACAGAGAAGCTG(+CAGTGT)GTACCAGGCTCTGTAGAGGTGGCACTCTGAG NGG 10 8 17 8 24 0 100 5 10
    240605174549_1hzs CTAAAGCAAGGGTTAGTCCTCAGACTCTGTAG(+CAGTGT)GATCCGCAGTAGATTTCTATAAAGACTAAAA NGG 10 8 17 8 24 0 100 5 10
    240605174548_69e6 TTTATTTTTAGTCTTTATAGAAATCTACTGCG(+CAGTGT)GATCCTACAGAGTCTGAGGACTAACCCTTGC NGG 10 8 17 8 24 0 100 5 10
    240605174546_wksa TGGGTGCACTTTGTCATGTAGTGGTCTCGGAG(+CAGTGT)GATCCGCCAGCAATGGAAAGGCTTGGCACTC NGG 10 8 17 8 24 0 100 5 10
    240605174544_dm00 ACACTGAGTGCCAAGCCTTTCCATTGCTGGCG(+CAGTGT)GATCCTCCGAGACCACTACATGACAAAGTGC NGG 10 8 17 8 24 0 100 5 10
    240605174543_7m8i ATGCTGTGGTAAGTACTACATCTACAATTTTG(+CAGTGT)GTACCCGAACTGGTGCTTTAGAAATGCGGGG NGG 10 8 17 8 24 0 100 5 10
    240605174542_4dle AAAAACCCCGCATTTCTAAAGCACCAGTTCGG(+CAGTGT)GTACCAAAATTGTAGATGTAGTACTTACCAC NGG 10 8 17 8 24 0 100 5 10
    240605174540_e7kj GCGTGTTCCAAAGATGGCTCCGTGTTGCAATG(+CAGTGT)GTACCCACCGCTTCACTAATGCCTCCAAGTG NGG 10 8 17 8 24 0 100 5 10
    240605174538_a3hl AGCCACACTTGGAGGCATTAGTGAAGCGGTGG(+CAGTGT)GTACCATTGCAACACGGAGCCATCTTTGGAA NGG 10 8 17 8 24 0 100 5 10
    240605174537_tj6d GTGCTTCATCCTAGAGAAGGCGACCGGTCTGG(+CAGTGT)GATCCATGCATATGGAAAGGCTGGTCTTACT NGG 10 8 17 8 24 0 100 5 10