Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605174536_59p8 ACCTGAGTAAGACCAGCCTTTCCATATGCATG(+CAGTGT)GATCCCAGACCGGTCGCCTTCTCTAGGATGA NGG 10 8 17 8 24 0 100 5 10
    240605174534_oeij GCAAACAGCAAGAGGCTTTATTGGGAGCACGG(+CAGTGT)GTACCCGGGCGACGCAGTCTATCGGAGGACT NGG 10 8 17 8 24 0 100 5 10
    240605174532_9qzm GCGCCAGTCCTCCGATAGACTGCGTCGCCCGG(+CAGTGT)GTACCCGTGCTCCCAATAAAGCCTCTTGCTG NGG 10 8 17 8 24 0 100 5 10
    240605174530_im0p CTGTGAACCAGCTATTCCGGTGTCATAGCTGG(+CAGTGT)GTACCTAACTTACTTCCATATGTGAAGTGTG NGG 10 8 17 8 24 0 100 5 10
    240605174529_m4x6 TTTAGCACACTTCACATATGGAAGTAAGTTAG(+CAGTGT)GTACCCAGCTATGACACCGGAATAGCTGGTT NGG 10 8 17 8 24 0 100 5 10
    240605174528_ws44 ACATTCATCTTGGTTTTCCTTCTTTATCACAG(+CAGTGT)GATCCAGCCTGGTGTTTCATGTATAGTAGGC NGG 10 8 17 8 24 0 100 5 10
    240605174526_4nv3 TCAGTGCCTACTATACATGAAACACCAGGCTG(+CAGTGT)GATCCTGTGATAAAGAAGGAAAACCAAGATG NGG 10 8 17 8 24 0 100 5 10
    240605174525_c4tq GAACCAGCAGATCCATAGCAAGGATCTCAGAG(+CAGTGT)GATCCTGGCTCATCCCAAGCAAAAGCAGGCA NGG 10 8 17 8 24 0 100 5 10
    240605174523_44dc ACGAATGCCTGCTTTTGCTTGGGATGAGCCAG(+CAGTGT)GATCCTCTGAGATCCTTGCTATGGATCTGCT NGG 10 8 17 8 24 0 100 5 10
    240605174522_72k8 AGCAGGGGGAGGGGTGGTTAGATCTGAAAATG(+CAGTGT)GTACCAGTGACTTCTTATCTCTGGGTGACTT NGG 10 8 17 8 24 0 100 5 10