Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605174521_xw29 TCCGGAAGTCACCCAGAGATAAGAAGTCACTG(+CAGTGT)GTACCATTTTCAGATCTAACCACCCCTCCCC NGG 10 8 17 8 24 0 100 5 10
    240605174519_1ovx ACTTGAATCTCACGTGGCAGGAGGGGCTATGG(+CAGTGT)GATCCGCTCGTCTTCCTCACTGCCGCACAGC NGG 10 8 17 8 24 0 100 5 10
    240605174518_dzdl GGGGAGCTGTGCGGCAGTGAGGAAGACGAGCG(+CAGTGT)GATCCCATAGCCCCTCCTGCCACGTGAGATT NGG 10 8 17 8 24 0 100 5 10
    240605174517_hwxn AATCAACCAATCATATGAAAAAGAACTTTGTG(+CAGTGT)GTACCTAGAGGGGCAGAGCCTGCAAGGTGGG NGG 10 8 17 8 24 0 100 5 10
    240605174516_jqhz CAGGGCCCACCTTGCAGGCTCTGCCCCTCTAG(+CAGTGT)GTACCACAAAGTTCTTTTTCATATGATTGGT NGG 10 8 17 8 24 0 100 5 10
    240605174514_3h83 CCCCTGGGAAAGAATTTTTTAAAAGGGCTAGG(+CAGTGT)GTACCATTCATCCAATATTTATAGAAAATGG NGG 10 8 17 8 24 0 100 5 10
    240605174513_upox TTCTGCCATTTTCTATAAATATTGGATGAATG(+CAGTGT)GTACCCTAGCCCTTTTAAAAAATTCTTTCCC NGG 10 8 17 8 24 0 100 5 10
    240605174511_g958 AATACACATGTGCCAAAACTTGTCTGTAGAGG(+CAGTGT)GATCCGACTCATGGGCCGTGTGCGTGCAGTA NGG 10 8 17 8 24 0 100 5 10
    240605174510_c9jt CTGCCTACTGCACGCACACGGCCCATGAGTCG(+CAGTGT)GATCCCTCTACAGACAAGTTTTGGCACATGT NGG 10 8 17 8 24 0 100 5 10
    240605174508_r7uj AATACACATGTGCCAAAACTTGTCTGTAGAGG(+CAGTGT)GATCCGACTCATGGGCCGTGTGCGTGCAGTA NGG 10 8 17 8 24 0 100 5 10