Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605174435_gisz ACCCATGCTGTAGCCTCTGCTGTAGGACTTTG(+CAGTGT)GTACCCAGTTGCCATAGAGGAGTGGGGTGCC NGG 10 8 17 8 24 0 100 5 10
    240605174434_fz9c GCCACACTCCACAGAAGCCTTCTCAGGAACTG(+CAGTGT)GATCCACAACCTAGCTACAGCCCCTCGCTGA NGG 10 8 17 8 24 0 100 5 10
    240605174433_1eki CCATCTCAGCGAGGGGCTGTAGCTAGGTTGTG(+CAGTGT)GATCCAGTTCCTGAGAAGGCTTCTGTGGAGT NGG 10 8 17 8 24 0 100 5 10
    240605174431_a9a7 GTAGTTGTCACCACCCTGGACGAGGGCCTTGG(+CAGTGT)GTACCGCTACCAGTGAGATAGGACTGGACTC NGG 10 8 17 8 24 0 100 5 10
    240605174429_vi1z GCTCGGAGTCCAGTCCTATCTCACTGGTAGCG(+CAGTGT)GTACCCAAGGCCCTCGTCCAGGGTGGTGACA NGG 10 8 17 8 24 0 100 5 10
    240605174428_i83b TGACTGGTGTGGCTGACCACCGTGGCAAGGAG(+CAGTGT)GATCCCTTGAAATACCAAGCTGATCCTACAG NGG 10 8 17 8 24 0 100 5 10
    240605174426_t7iz AGCCACTGTAGGATCAGCTTGGTATTTCAAGG(+CAGTGT)GATCCTCCTTGCCACGGTGGTCAGCCACACC NGG 10 8 17 8 24 0 100 5 10
    240605174424_4zc9 ATACACCACAGATATCGACAGGTACTCCATCG(+CAGTGT)GTACCACTAAAGGAATGTTTCCCACTGCCTG NGG 10 8 17 8 24 0 100 5 10
    240605174423_e7i4 AACCCCAGGCAGTGGGAAACATTCCTTTAGTG(+CAGTGT)GTACCGATGGAGTACCTGTCGATATCTGTGG NGG 10 8 17 8 24 0 100 5 10
    240605174422_5ixh CCAGCAGAGAACAAGCAGCCATGAGTCGGCTG(+CAGTGT)GATCCTGTGCTAATGTGACGCTCCAAACTTA NGG 10 8 17 8 24 0 100 5 10