Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605174333_ckil TAGCTATCTTCAGACACCAGAACAGGGCATCG(+CAGTGT)GATCCCATTACAGATGGTTCTGAGCCACCAT NGG 10 8 17 8 24 0 100 5 10
    240605174332_hhli TACCCCAAAAAGGTGAGGTGTCCACAGCTGGG(+CAGTGT)GTACCACCAGTATACAGACCTTTAGAATTTG NGG 10 8 17 8 24 0 100 5 10
    240605174330_aowt ATGGACAAATTCTAAAGGTCTGTATACTGGTG(+CAGTGT)GTACCCCAGCTGTGGACACCTCACCTTTTTG NGG 10 8 17 8 24 0 100 5 10
    240605174328_1pqk ACCAACTTTTAGGTTGTGATACTTTCCTCCTG(+CAGTGT)GTACCTGGGGGGAGGGGAGTATGCAGTTGGT NGG 10 8 17 8 24 0 100 5 10
    240605174325_mu9v AAAGCACCAACTGCATACTCCCCTCCCCCCAG(+CAGTGT)GTACCAGGAGGAAAGTATCACAACCTAAAAG NGG 10 8 17 8 24 0 100 5 10
    240605174324_y8s0 TTTCTTAGCTCTAGACCCTTCTGGTCTCTTTG(+CAGTGT)GTACCTAGAACAATGTTTTATATTTACAGCT NGG 10 8 17 8 24 0 100 5 10
    240605174323_k61c TAAACAGCTGTAAATATAAAACATTGTTCTAG(+CAGTGT)GTACCAAAGAGACCAGAAGGGTCTAGAGCTA NGG 10 8 17 8 24 0 100 5 10
    240605174322_oq0m CAGGGTGAGGTGCCTGCTGGGCACAGACTGTG(+CAGTGT)GATCCACTGTCTTCCTAGAGCAGGCCACATG NGG 10 8 17 8 24 0 100 5 10
    240605174321_qxjo AAGGCCATGTGGCCTGCTCTAGGAAGACAGTG(+CAGTGT)GATCCACAGTCTGTGCCCAGCAGGCACCTCA NGG 10 8 17 8 24 0 100 5 10
    240605174319_jsad CTCTACCTCCCAGTTTCTGGGGTTACAAGTAG(+CAGTGT)GTACCATCACACACAATTTCTGTACTTTAAG NGG 10 8 17 8 24 0 100 5 10