Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605174230_b922 CCAGAGTTAGGGATGTGATTTTTGTCTGTGAG(+CAGTGT)GTACCCCTTGCCCTAGTGGATCAGTTACAGG NGG 10 8 17 8 24 0 100 5 10
    240605174228_pv4g CGGGATTGTTGCCTCTGTCTCCAACACCCTTG(+CAGTGT)GATCCTACCGATCCGTGGGCACACGTCCATA NGG 10 8 17 8 24 0 100 5 10
    240605174226_nl1c AGGATTATGGACGTGTGCCCACGGATCGGTAG(+CAGTGT)GATCCAAGGGTGTTGGAGACAGAGGCAACAA NGG 10 8 17 8 24 0 100 5 10
    240605174225_uaf8 TCCCCAGTTGCCAACTCACTGCAGGTGACACG(+CAGTGT)GTACCTCAGGAAGTGTAAGGACCCTTCTCCT NGG 10 8 17 8 24 0 100 5 10
    240605174223_ss0k GGTGGAGGAGAAGGGTCCTTACACTTCCTGAG(+CAGTGT)GTACCGTGTCACCTGCAGTGAGTTGGCAACT NGG 10 8 17 8 24 0 100 5 10
    240605174222_cjk3 GTCAGCTCTTTCCTTTCACATGTGTGATTCAG(+CAGTGT)GTACCTTTACCTGCTGAGCAAGTCCTAAACT NGG 10 8 17 8 24 0 100 5 10
    240605174221_9zfl TAAGAAGTTTAGGACTTGCTCAGCAGGTAAAG(+CAGTGT)GTACCTGAATCACACATGTGAAAGGAAAGAG NGG 10 8 17 8 24 0 100 5 10
    240605174219_yrrw TATTCATTTGGAGGTTCTTAATTTGAAGATGG(+CAGTGT)GATCCAGGTTTGGACTGACTGGAGATATTTG NGG 10 8 17 8 24 0 100 5 10
    240605174217_wp99 ATCACCAAATATCTCCAGTCAGTCCAAACCTG(+CAGTGT)GATCCCATCTTCAAATTAAGAACCTCCAAAT NGG 10 8 17 8 24 0 100 5 10
    240605174216_abo2 TCCTTATAGCACTCAAGTGGCAGAGGCAGAAG(+CAGTGT)GATCCCTAAGAATTTGAACCAGACTAGGTTA NGG 10 8 17 8 24 0 100 5 10