Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605174155_vnf6 AGACCAGGGCTTGGGCCCTTGGGTTAACCCAG(+CAGTGT)GTACCCCTGGCTGCATCTTTCAGGGCAAGGT NGG 10 8 17 8 24 0 100 5 10
    240605174154_4js6 TGGGTGAGGAGGTACACACAGGGCTCAGGATG(+CAGTGT)GATCCCCTATGGGGAGGAGGGCAGACAATTT NGG 10 8 17 8 24 0 100 5 10
    240605174152_8s8w AGTGCAAATTGTCTGCCCTCCTCCCCATAGGG(+CAGTGT)GATCCATCCTGAGCCCTGTGTGTACCTCCTC NGG 10 8 17 8 24 0 100 5 10
    240605174150_9wvr TGGGAATCACACTTTAAAAGGGTCAGAATATG(+CAGTGT)GATCCCGAGGGAAAGAATGCTCCGCACTGTT NGG 10 8 17 8 24 0 100 5 10
    240605174148_1h4x TCATGAACAGTGCGGAGCATTCTTTCCCTCGG(+CAGTGT)GATCCATATTCTGACCCTTTTAAAGTGTGAT NGG 10 8 17 8 24 0 100 5 10
    240605174147_t576 TGCAATGCCTCAGAGACAAGAAGAGTGCATTG(+CAGTGT)GATCCTAGGGGGCTGCAGTTACAGTCTGTTG NGG 10 8 17 8 24 0 100 5 10
    240605174145_oc51 ACTCACAACAGACTGTAACTGCAGCCCCCTAG(+CAGTGT)GATCCAATGCACTCTTCTTGTCTCTGAGGCA NGG 10 8 17 8 24 0 100 5 10
    240605174143_pp8y TGCAATATTTGTACACATATACAAGTCCTGAG(+CAGTGT)GATCCTAATTTCAGCTCAGAGATCGAATTGG NGG 10 8 17 8 24 0 100 5 10
    240605174142_a0ic CTGACCCAATTCGATCTCTGAGCTGAAATTAG(+CAGTGT)GATCCTCAGGACTTGTATATGTGTACAAATA NGG 10 8 17 8 24 0 100 5 10
    240605174140_cb8g CAAGGAGTTCTAGCATACACTTTTGTGATTGG(+CAGTGT)GTACCATATGGTTATATCTGATTCTTGGTAG NGG 10 8 17 8 24 0 100 5 10