Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605174214_ti5o CTATGTAACCTAGTCTGGTTCAAATTCTTAGG(+CAGTGT)GATCCTTCTGCCTCTGCCACTTGAGTGCTAT NGG 10 8 17 8 24 0 100 5 10
    240605174213_dwji AAGTCCTCACAGAGGACACAGCTCAGGATCGG(+CAGTGT)GATCCCACACCTGTAAAAAGTAAAGAACAAG NGG 10 8 17 8 24 0 100 5 10
    240605174211_nrww AACCACTTGTTCTTTACTTTTTACAGGTGTGG(+CAGTGT)GATCCCGATCCTGAGCTGTGTCCTCTGTGAG NGG 10 8 17 8 24 0 100 5 10
    240605174209_tivt TGACACCTGATACCAGCAGGAGGTATACTTGG(+CAGTGT)GATCCAATATGAATCAGTGGATTGCTCTGGT NGG 10 8 17 8 24 0 100 5 10
    240605174208_g1tm CTAGAACCAGAGCAATCCACTGATTCATATTG(+CAGTGT)GATCCCAAGTATACCTCCTGCTGGTATCAGG NGG 10 8 17 8 24 0 100 5 10
    240605174206_bcvp AACTGCAGACTGGAGAGACCTGCACTTCTGGG(+CAGTGT)GATCCTGCACCGACCTACAGCTTCCTTGAAG NGG 10 8 17 8 24 0 100 5 10
    240605174205_p1se AATTTCTTCAAGGAAGCTGTAGGTCGGTGCAG(+CAGTGT)GATCCCCAGAAGTGCAGGTCTCTCCAGTCTG NGG 10 8 17 8 24 0 100 5 10
    240605174203_m75g AGACTCTTCCAGGGGAGGGGTGCCAGGATGGG(+CAGTGT)GTACCCCTGGTCACTCACCAGGATCACTGTA NGG 10 8 17 8 24 0 100 5 10
    240605174200_u52n AGGGTTACAGTGATCCTGGTGAGTGACCAGGG(+CAGTGT)GTACCCCATCCTGGCACCCCTCCCCTGGAAG NGG 10 8 17 8 24 0 100 5 10
    240605174157_4ilz TGGTTACCTTGCCCTGAAAGATGCAGCCAGGG(+CAGTGT)GTACCTGGGTTAACCCAAGGGCCCAAGCCCT NGG 10 8 17 8 24 0 100 5 10