Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605174111_h3vy ATTTCATTTATTCCTGTGGATATTTCTTGCTG(+CAGTGT)GTACCAATGGTACTGTACAAAGTGAATGTTC NGG 10 8 17 8 24 0 100 5 10
    240605174110_esjm TATCTTCAGGAGATATGTTACTGCACTTGCAG(+CAGTGT)GTACCTTAATTCACTTGAACATTTACAAAGG NGG 10 8 17 8 24 0 100 5 10
    240605174109_bzff AGTGTCCTTTGTAAATGTTCAAGTGAATTAAG(+CAGTGT)GTACCTGCAAGTGCAGTAACATATCTCCTGA NGG 10 8 17 8 24 0 100 5 10
    240605174107_3plz ATAAACAACAAAGCTAGTCATTTTGTCCTATG(+CAGTGT)GATCCTGTCCTCACATTAAACACTGTCCAGT NGG 10 8 17 8 24 0 100 5 10
    240605174106_tjcm CCGCCACTGGACAGTGTTTAATGTGAGGACAG(+CAGTGT)GATCCATAGGACAAAATGACTAGCTTTGTTG NGG 10 8 17 8 24 0 100 5 10
    240605174104_gpyr ACCCTTTTCTTAGGGCACACAAATTAATCTAG(+CAGTGT)GTACCTTGTTGTGTACAATTGAGAACACCTT NGG 10 8 17 8 24 0 100 5 10
    240605174103_m7r4 AAGTCAAGGTGTTCTCAATTGTACACAACAAG(+CAGTGT)GTACCTAGATTAATTTGTGTGCCCTAAGAAA NGG 10 8 17 8 24 0 100 5 10
    240605174102_z6cq AAAGCAAATGGCAGAGCCTTTTGATATCAGAG(+CAGTGT)GATCCTCTTACACTTCTTCCCCCCTCCATTG NGG 10 8 17 8 24 0 100 5 10
    240605174101_k8a5 CAGCACAATGGAGGGGGGAAGAAGTGTAAGAG(+CAGTGT)GATCCTCTGATATCAAAAGGCTCTGCCATTT NGG 10 8 17 8 24 0 100 5 10
    240605174059_x910 TACATAGTTCAGAGTTGCTTCTGGGGGATCTG(+CAGTGT)GTACCTTTAATATTTAAACTTTGAGCAATAT NGG 10 8 17 8 24 0 100 5 10