Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605174043_eqp5 GGAAGAGTGAGTCCGGCCAGTTCATTCTTTAG(+CAGTGT)GATCCTCTGTTACTGCAGGCCTCCCCTGCTA NGG 10 8 17 8 24 0 100 5 10
    240605174042_66h2 CACTCCTCCATTGCTGGTGGGATTGCAAGCTG(+CAGTGT)GTACCATCACTCTGGAAATCAGTCTTGCAGT NGG 10 8 17 8 24 0 100 5 10
    240605174041_eioz TAGGAACTGCAAGACTGATTTCCAGAGTGATG(+CAGTGT)GTACCAGCTTGCAATCCCACCAGCAATGGAG NGG 10 8 17 8 24 0 100 5 10
    240605174040_qiqg TAACTTTATTTTAAAAAGAAAACAAAGCTTTG(+CAGTGT)GATCCCAAACCACAGATTAATGGCTACACAG NGG 10 8 17 8 24 0 100 5 10
    240605174038_ny48 AATCACTGTGTAGCCATTAATCTGTGGTTTGG(+CAGTGT)GATCCAAAGCTTTGTTTTCTTTTTAAAATAA NGG 10 8 17 8 24 0 100 5 10
    240605174036_sfvm TCAACTAGTCTTCCTTGTAATCAGAGAAACCG(+CAGTGT)GATCCACCAAGTCTTACTCAGCATCCAGCGA NGG 10 8 17 8 24 0 100 5 10
    240605174034_4qkj ACCAATCGCTGGATGCTGAGTAAGACTTGGTG(+CAGTGT)GATCCGGTTTCTCTGATTACAAGGAAGACTA NGG 10 8 17 8 24 0 100 5 10
    240605174032_hkt8 AGTGTTTAACAGCACTGGCTGCTCTTCCAGAG(+CAGTGT)GTACCCAGGCTTAATTCCCAGCACCAACATG NGG 10 8 17 8 24 0 100 5 10
    240605174030_qxqk ACTGTCATGTTGGTGCTGGGAATTAAGCCTGG(+CAGTGT)GTACCTCTGGAAGAGCAGCCAGTGCTGTTAA NGG 10 8 17 8 24 0 100 5 10
    240605174029_eqng CTAGTTTTATGTCAACATGGTACAAGCTAGAG(+CAGTGT)GTACCAGAGAGGAGGGAGCCTCAATTGGGAA NGG 10 8 17 8 24 0 100 5 10