Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605173956_pmtk ACACTTGTTACTGAAGACTGGTTTCAAGTCTG(+CAGTGT)GTACCCAGTGATGCCTTCCGAACGGCCTGGA NGG 10 8 17 8 24 0 100 5 10
    240605173954_mx2m CCGTGGGATTATAGGAAAACCAGGGCCTCTTG(+CAGTGT)GTACCCTGTCCTCAGAGTGTGTGACAGCCAC NGG 10 8 17 8 24 0 100 5 10
    240605173953_ap1g TTACAGTGGCTGTCACACACTCTGAGGACAGG(+CAGTGT)GTACCAAGAGGCCCTGGTTTTCCTATAATCC NGG 10 8 17 8 24 0 100 5 10
    240605173952_fpok GCACCTCCGAAGCTCTTAGTAATGTTGTGCTG(+CAGTGT)GATCCTGTAAGTTCGCTCTGCAGTCACTCGC NGG 10 8 17 8 24 0 100 5 10
    240605173951_bfxm GGTTTGCGAGTGACTGCAGAGCGAACTTACAG(+CAGTGT)GATCCAGCACAACATTACTAAGAGCTTCGGA NGG 10 8 17 8 24 0 100 5 10
    240605173949_sajg TGATGCTCCACAAACTGTCTCATTAATCCATG(+CAGTGT)GATCCTAAAACAAATTCTCTTTGTGTATTGT NGG 10 8 17 8 24 0 100 5 10
    240605173948_dvgw CACACACAATACACAAAGAGAATTTGTTTTAG(+CAGTGT)GATCCATGGATTAATGAGACAGTTTGTGGAG NGG 10 8 17 8 24 0 100 5 10
    240605173946_6049 TCTTCCTTTTGAAGCTAGGCTGTGCTGTGTGG(+CAGTGT)GATCCAGTGGTCTGTTCTTTACTGGAGGTAT NGG 10 8 17 8 24 0 100 5 10
    240605173944_2te4 AACACATACCTCCAGTAAAGAACAGACCACTG(+CAGTGT)GATCCCACACAGCACAGCCTAGCTTCAAAAG NGG 10 8 17 8 24 0 100 5 10
    240605173943_qkat AGTAAAACCTTTAAACATGGTGACTATCTTCG(+CAGTGT)GATCCCAAGGAGAATATTAACAGGCACCAGG NGG 10 8 17 8 24 0 100 5 10