Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605173849_a8fu AAAATGGGGTTTTTACAGCTTTTGACTGCCTG(+CAGTGT)GTACCCCATGTTGTTCCCTTTAGTGACAAAG NGG 10 8 17 8 24 0 100 5 10
    240605173848_u4dm TCTCTAGCCAGCCCTGCTGCTCCTGTTGTCTG(+CAGTGT)GTACCCAGGTGACTGAGGACATGACTGGTGG NGG 10 8 17 8 24 0 100 5 10
    240605173845_5i1e TCCTTCCACCAGTCATGTCCTCAGTCACCTGG(+CAGTGT)GTACCAGACAACAGGAGCAGCAGGGCTGGCT NGG 10 8 17 8 24 0 100 5 10
    240605173843_1j5s GTGCACAGTTCTGTGAGGCTTTGGGGCAAGGG(+CAGTGT)GTACCAAGAGCCCAAGAGAGCCTTTCTTGTT NGG 10 8 17 8 24 0 100 5 10
    240605173842_73zr TTTAGAACAAGAAAGGCTCTCTTGGGCTCTTG(+CAGTGT)GTACCCCTTGCCCCAAAGCCTCACAGAACTG NGG 10 8 17 8 24 0 100 5 10
    240605173841_2sjc TAGGGAGGGATGTCAAGGCCAAGAGTCATACG(+CAGTGT)GTACCCAGTAACTGCTGGTGTAGCTCTTGGG NGG 10 8 17 8 24 0 100 5 10
    240605173839_5c9i TGCCTCCCAAGAGCTACACCAGCAGTTACTGG(+CAGTGT)GTACCGTATGACTCTTGGCCTTGACATCCCT NGG 10 8 17 8 24 0 100 5 10
    240605173837_2tdm CCAACAGGGAGGGACCAAGGGCAGCCACCAGG(+CAGTGT)GATCCTATCTCACCTCCGATGCCTGCTGTGT NGG 10 8 17 8 24 0 100 5 10
    240605173836_uk26 CCTGGACACAGCAGGCATCGGAGGTGAGATAG(+CAGTGT)GATCCCTGGTGGCTGCCCTTGGTCCCTCCCT NGG 10 8 17 8 24 0 100 5 10
    240605173834_1c82 CATGGGCTAGTTTCCATTAGGCCCTCCATGGG(+CAGTGT)GATCCTAAAGAGGGAGAGGGAATGAGAGAAA NGG 10 8 17 8 24 0 100 5 10