Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605173816_vwfd CTCCCTGGCCCCTTCTCCATCTCCTGCAGCTG(+CAGTGT)GTACCCTGGCTTTGGACTCTGGCCCTGGGGC NGG 10 8 17 8 24 0 100 5 10
    240605173814_t6bi ACCGTGTGATAAGGTTGTGTGCTGTGTGATCG(+CAGTGT)GTACCCAGGGAGCGGGGCGGTGTTAATGTGC NGG 10 8 17 8 24 0 100 5 10
    240605173811_4pjy ATTTGGCACATTAACACCGCCCCGCTCCCTGG(+CAGTGT)GTACCGATCACACAGCACACAACCTTATCAC NGG 10 8 17 8 24 0 100 5 10
    240605173809_7x38 TTGCTGATGCTCAGTGGTCCAGGCTACGCTGG(+CAGTGT)GATCCGTGATGGGGATATCCCAGAAGACACC NGG 10 8 17 8 24 0 100 5 10
    240605173808_mr0z GGAATGGTGTCTTCTGGGATATCCCCATCACG(+CAGTGT)GATCCCAGCGTAGCCTGGACCACTGAGCATC NGG 10 8 17 8 24 0 100 5 10
    240605173807_hv3d ACAGCAATCCATGGCTTTCCAGAAGAGGTGTG(+CAGTGT)GATCCAAAAGTGCCACCTCTATCTTAGTCAC NGG 10 8 17 8 24 0 100 5 10
    240605173806_6k7r TGCTGGTGACTAAGATAGAGGTGGCACTTTTG(+CAGTGT)GATCCACACCTCTTCTGGAAAGCCATGGATT NGG 10 8 17 8 24 0 100 5 10
    240605173804_hq4o TCAAGTGCCAAAGCTGTCTCGTGAGCTTCCAG(+CAGTGT)GATCCTGACAGGCCTGGAGGGAACTGCAAAG NGG 10 8 17 8 24 0 100 5 10
    240605173803_h8uq TGGCCCTTTGCAGTTCCCTCCAGGCCTGTCAG(+CAGTGT)GATCCTGGAAGCTCACGAGACAGCTTTGGCA NGG 10 8 17 8 24 0 100 5 10
    240605173801_utcm CAGGTGTTCTTTCAGGCTGGTCAGGAGCTGTG(+CAGTGT)GTACCAGGGACAAGGCCTGCCTCTTGTCTTT NGG 10 8 17 8 24 0 100 5 10