Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605173727_178s GGCAGGCATGAATCTGCCAAATACTGGATATG(+CAGTGT)GATCCAAGGCTGGCCCAGGCACCTGCACCTC NGG 10 8 17 8 24 0 100 5 10
    240605173726_cbju TGCCCATCGCTAAAGCTAAGACCTGCAGTAAG(+CAGTGT)GATCCCTCAGAGCCTCTTTTCAAAGCATGGT NGG 10 8 17 8 24 0 100 5 10
    240605173724_s1ux GAGCAACCATGCTTTGAAAAGAGGCTCTGAGG(+CAGTGT)GATCCTTACTGCAGGTCTTAGCTTTAGCGAT NGG 10 8 17 8 24 0 100 5 10
    240605173723_pdio AAAAAGCTACAAACATTTCCAACAGACAAATG(+CAGTGT)GATCCTAGCTGCCCCATGCATGGTTCTGGGA NGG 10 8 17 8 24 0 100 5 10
    240605173722_5q0b CATGCTCCCAGAACCATGCATGGGGCAGCTAG(+CAGTGT)GATCCATTTGTCTGTTGGAAATGTTTGTAGC NGG 10 8 17 8 24 0 100 5 10
    240605173720_98j5 TAGGGGCTGGAGCTGAGCTGCCTTCTGTCCTG(+CAGTGT)GTACCTGGAGGGAACGAGGATGTTAAGTGAT NGG 10 8 17 8 24 0 100 5 10
    240605173718_yb19 AGTCAATCACTTAACATCCTCGTTCCCTCCAG(+CAGTGT)GTACCAGGACAGAAGGCAGCTCAGCTCCAGC NGG 10 8 17 8 24 0 100 5 10
    240605173716_3i78 CTGCCCACTGTGCCTCAGCTGTGTGGAGCCTG(+CAGTGT)GTACCAGAGTCAGGCCCCAGAGAAGGGAGGA NGG 10 8 17 8 24 0 100 5 10
    240605173715_kgmk TTTTGTCCTCCCTTCTCTGGGGCCTGACTCTG(+CAGTGT)GTACCAGGCTCCACACAGCTGAGGCACAGTG NGG 10 8 17 8 24 0 100 5 10
    240605173713_jxa2 GGGCATCTCCACCATTCTTACTGACGAACCTG(+CAGTGT)GTACCACTGGGAACACTACAGGCAGCTCCTA NGG 10 8 17 8 24 0 100 5 10