Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605173833_7tzn CTGCTTTTCTCTCATTCCCTCTCCCTCTTTAG(+CAGTGT)GATCCCCATGGAGGGCCTAATGGAAACTAGC NGG 10 8 17 8 24 0 100 5 10
    240605173831_6alk GCTCATAATTATCTGTAACTCCAATTTCAGGG(+CAGTGT)GATCCAACACCCTCATAAAGACACACATGCA NGG 10 8 17 8 24 0 100 5 10
    240605173829_980l TTGCCTGCATGTGTGTCTTTATGAGGGTGTTG(+CAGTGT)GATCCCCTGAAATTGGAGTTACAGATAATTA NGG 10 8 17 8 24 0 100 5 10
    240605173828_andj AGTGTCTGGGTTTGGTGGTTGTTTATGGGATG(+CAGTGT)GATCCCCAGGTGGGGCAGTCTTTGGATGGTC NGG 10 8 17 8 24 0 100 5 10
    240605173826_eacj GGAAGGACCATCCAAAGACTGCCCCACCTGGG(+CAGTGT)GATCCATCCCATAAACAACCACCAAACCCAG NGG 10 8 17 8 24 0 100 5 10
    240605173824_qly8 GGATTAAGGTACTATGCCAAGCGTTTAAAGTG(+CAGTGT)GATCCTGTGGATAAACTTAGCTTCAAGAACA NGG 10 8 17 8 24 0 100 5 10
    240605173823_3j4y ACCTGTGTTCTTGAAGCTAAGTTTATCCACAG(+CAGTGT)GATCCACTTTAAACGCTTGGCATAGTACCTT NGG 10 8 17 8 24 0 100 5 10
    240605173821_hssl TGAGGTGTTCTGGAGGGAATGGGCATGACAGG(+CAGTGT)GTACCTTAGGCTGAAAGCCAGCAAAGGCACA NGG 10 8 17 8 24 0 100 5 10
    240605173819_xflk CATCCTGTGCCTTTGCTGGCTTTCAGCCTAAG(+CAGTGT)GTACCCTGTCATGCCCATTCCCTCCAGAACA NGG 10 8 17 8 24 0 100 5 10
    240605173817_7am0 TGGAGGCCCCAGGGCCAGAGTCCAAAGCCAGG(+CAGTGT)GTACCAGCTGCAGGAGATGGAGAAGGGGCCA NGG 10 8 17 8 24 0 100 5 10