Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605173940_5zg2 GAGTTCCTGGTGCCTGTTAATATTCTCCTTGG(+CAGTGT)GATCCGAAGATAGTCACCATGTTTAAAGGTT NGG 10 8 17 8 24 0 100 5 10
    240605173939_vzbv GTAAAAAAAAAAAAAAAGGCAGATGGCAGATG(+CAGTGT)GATCCTGGAGCTTTTTCTCTCTTTTTTTTTC NGG 10 8 17 8 24 0 100 5 10
    240605173937_i9k0 CTGGGGAAAAAAAAAGAGAGAAAAAGCTCCAG(+CAGTGT)GATCCATCTGCCATCTGCCTTTTTTTTTTTT NGG 10 8 17 8 24 0 100 5 10
    240605173935_6km2 CGAAGAAAAGTGCCTCTGTGTACTCTCCCTCG(+CAGTGT)GATCCAGTTTTACCCTCAGCTGCATGAAAAC NGG 10 8 17 8 24 0 100 5 10
    240605173934_ern2 CAAACGTTTTCATGCAGCTGAGGGTAAAACTG(+CAGTGT)GATCCGAGGGAGAGTACACAGAGGCACTTTT NGG 10 8 17 8 24 0 100 5 10
    240605173931_iwgr AGTGTGAGCAACCACTTCTGTGTTTACCTGGG(+CAGTGT)GATCCATCCCACAATCAGCCTCCAAACGCTG NGG 10 8 17 8 24 0 100 5 10
    240605173929_xsal GGTGTCAGCGTTTGGAGGCTGATTGTGGGATG(+CAGTGT)GATCCCCAGGTAAACACAGAAGTGGTTGCTC NGG 10 8 17 8 24 0 100 5 10
    240605173926_x92z GGATGGACCATCCAGAGACTGCCCCACCCGGG(+CAGTGT)GATCCATCCCATAATCAGCCACCAAATGCAG NGG 10 8 17 8 24 0 100 5 10
    240605173925_iuqt AGTGTCTGCATTTGGTGGCTGATTATGGGATG(+CAGTGT)GATCCCCGGGTGGGGCAGTCTCTGGATGGTC NGG 10 8 17 8 24 0 100 5 10
    240605173923_feta CAGGGCCAAGAAGTAGGAGTGGGTGAGTAGGG(+CAGTGT)GATCCGGGTGGAGGTATAGGGGACTTTCAGG NGG 10 8 17 8 24 0 100 5 10