Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605174010_q0r1 GAGTGGCAATGGCCCACAAACACAGATAGAAG(+CAGTGT)GTACCCTGAATTCTGTTTCAATGCACAAGCA NGG 10 8 17 8 24 0 100 5 10
    240605174009_ywkz ATAGCATCCTGGTGAAATGAGCTATGTCTCAG(+CAGTGT)GTACCACTAAACATCAGCCACTCACCTTTAG NGG 10 8 17 8 24 0 100 5 10
    240605174008_bu6x CTGGCCTAAAGGTGAGTGGCTGATGTTTAGTG(+CAGTGT)GTACCTGAGACATAGCTCATTTCACCAGGAT NGG 10 8 17 8 24 0 100 5 10
    240605174006_0q72 CTTTATGGTCAAGAGATTATTATGACACAGGG(+CAGTGT)GTACCATTAGCGTAACAGCCCTCCAGAGAGT NGG 10 8 17 8 24 0 100 5 10
    240605174005_dy8q TGAGCACTCTCTGGAGGGCTGTTACGCTAATG(+CAGTGT)GTACCCCTGTGTCATAATAATCTCTTGACCA NGG 10 8 17 8 24 0 100 5 10
    240605174003_jqsn GGCACATGGGTCAGAACAAAGTTGCATTATGG(+CAGTGT)GTACCAGCGATCCAATTAATTAATATTTATA NGG 10 8 17 8 24 0 100 5 10
    240605174002_pm31 CCAGCTATAAATATTAATTAATTGGATCGCTG(+CAGTGT)GTACCCATAATGCAACTTTGTTCTGACCCAT NGG 10 8 17 8 24 0 100 5 10
    240605174001_cb5h GTCATGGGCACTGTGGCTGAAGTGGCTGCTTG(+CAGTGT)GTACCAGCCGTCTCAGTAGCAGGAGTGAGCC NGG 10 8 17 8 24 0 100 5 10
    240605173959_567y CTTGTGGCTCACTCCTGCTACTGAGACGGCTG(+CAGTGT)GTACCAAGCAGCCACTTCAGCCACAGTGCCC NGG 10 8 17 8 24 0 100 5 10
    240605173957_d5z2 AACATTCCAGGCCGTTCGGAAGGCATCACTGG(+CAGTGT)GTACCAGACTTGAAACCAGTCTTCAGTAACA NGG 10 8 17 8 24 0 100 5 10