Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605173642_q53i CAGCCCTGACCTTGCTTGGAGAAGGTGGCAGG(+CAGTGT)GTACCAGACATTGCCACTAGCAGCTCCTGGA NGG 10 8 17 8 24 0 100 5 10
    240605173640_i344 ATAAGCTCCTGTCCCTGGGGACTAGGCATTAG(+CAGTGT)GATCCCAGCATACTTGGGAGGCAGGGTCATT NGG 10 8 17 8 24 0 100 5 10
    240605173639_dog2 TGTGTAATGACCCTGCCTCCCAAGTATGCTGG(+CAGTGT)GATCCTAATGCCTAGTCCCCAGGGACAGGAG NGG 10 8 17 8 24 0 100 5 10
    240605173637_0y94 GGAAGCAGGCTGGCTCCGACCCAGGGCCTCAG(+CAGTGT)GATCCCCTTGTGTACAGGGTCCATGAAACTG NGG 10 8 17 8 24 0 100 5 10
    240605173635_y2qd GTGGTCAGTTTCATGGACCCTGTACACAAGGG(+CAGTGT)GATCCTGAGGCCCTGGGTCGGAGCCAGCCTG NGG 10 8 17 8 24 0 100 5 10
    240605173634_14ho ACTTTGAGGCAGGCACAATGCTAGGAGCGCGG(+CAGTGT)GATCCAGCAGCAGCCCCATACCCCCTCGGAT NGG 10 8 17 8 24 0 100 5 10
    240605173632_lqn2 GTACGATCCGAGGGGGTATGGGGCTGCTGCTG(+CAGTGT)GATCCCGCGCTCCTAGCATTGTGCCTGCCTC NGG 10 8 17 8 24 0 100 5 10
    240605173631_rwi8 CGCTCTCTGCTTTGGCTTTAAAGTCAGGCCTG(+CAGTGT)GTACCAGATAGGCAGGATCAGACTATCTTTG NGG 10 8 17 8 24 0 100 5 10
    240605173629_5h4y GATGACAAAGATAGTCTGATCCTGCCTATCTG(+CAGTGT)GTACCAGGCCTGACTTTAAAGCCAAAGCAGA NGG 10 8 17 8 24 0 100 5 10
    240605173628_8ezq TCCCTTCAACTTTCCCTAGTCTACATCTGCAG(+CAGTGT)GTACCAATAAGACCATCTACATAACCTGCAA NGG 10 8 17 8 24 0 100 5 10