Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605174302_c698 CAAGTGACTTTACCCACTGACCCATCTCTCTG(+CAGTGT)GATCCTCAAATGTCTTTTTAAACAAAACAAC NGG 10 8 17 8 24 0 100 5 10
    240605174300_hxnq AGTGCTCAGGGGCCTACTGGACATTGACCCAG(+CAGTGT)GTACCCACTGGAGAAAGCTGCACCCACTCAG NGG 10 8 17 8 24 0 100 5 10
    240605174258_ik23 GTTTGCTGAGTGGGTGCAGCTTTCTCCAGTGG(+CAGTGT)GTACCTGGGTCAATGTCCAGTAGGCCCCTGA NGG 10 8 17 8 24 0 100 5 10
    240605174257_4i5f CTGGGATGGGAGCCACCTTGTTTGGTGATCAG(+CAGTGT)GATCCTGGTGAACTTGTTAGTAAGTGCTCAG NGG 10 8 17 8 24 0 100 5 10
    240605174255_8ofg GGCCCCTGAGCACTTACTAACAAGTTCACCAG(+CAGTGT)GATCCTGATCACCAAACAAGGTGGCTCCCAT NGG 10 8 17 8 24 0 100 5 10
    240605174253_dlb2 CTGGGTCTTGGGAGCTCTTGCCTTCACCTGTG(+CAGTGT)GATCCACTTAAGACATTGTGGTCTGAAGCTC NGG 10 8 17 8 24 0 100 5 10
    240605174252_z31l GGCCTGAGCTTCAGACCACAATGTCTTAAGTG(+CAGTGT)GATCCACAGGTGAAGGCAAGAGCTCCCAAGA NGG 10 8 17 8 24 0 100 5 10
    240605174251_n2nx GATCTTGCCTGTGAGATCCAGAAGAAGTGTTG(+CAGTGT)GATCCTCTGGAGCTGGAGTTAGAGGTAGCTG NGG 10 8 17 8 24 0 100 5 10
    240605174249_7yvv TCTCTCAGCTACCTCTAACTCCAGCTCCAGAG(+CAGTGT)GATCCAACACTTCTTCTGGATCTCACAGGCA NGG 10 8 17 8 24 0 100 5 10
    240605174248_6h43 CCGACATTCCAAGGAGCTATGGTTGTCACATG(+CAGTGT)GTACCAGCAGCTGGAAGACGGGACTAACGTA NGG 10 8 17 8 24 0 100 5 10