Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605174246_vp9y AATGATACGTTAGTCCCGTCTTCCAGCTGCTG(+CAGTGT)GTACCATGTGACAACCATAGCTCCTTGGAAT NGG 10 8 17 8 24 0 100 5 10
    240605174245_07xz AAGTCTGTCATCCTGTGGATGCTATGGGTCTG(+CAGTGT)GATCCTGGGAGCGGGGCCACACTGGCTCTGA NGG 10 8 17 8 24 0 100 5 10
    240605174243_5iqz GAAACTCAGAGCCAGTGTGGCCCCGCTCCCAG(+CAGTGT)GATCCAGACCCATAGCATCCACAGGATGACA NGG 10 8 17 8 24 0 100 5 10
    240605174241_z6o8 GGGCAGCACGACCCATACCATGGAAAACAGGG(+CAGTGT)GATCCAGTGTGGGTTCCTCAATGGCCCCTAT NGG 10 8 17 8 24 0 100 5 10
    240605174239_e28n AGAGGATAGGGGCCATTGAGGAACCCACACTG(+CAGTGT)GATCCCCTGTTTTCCATGGTATGGGTCGTGC NGG 10 8 17 8 24 0 100 5 10
    240605174238_64w6 GCAGTTTTGTGGCAGAATGGGCCATTGTTGTG(+CAGTGT)GATCCATCCAAACGTATCCCCAGCTAAAATA NGG 10 8 17 8 24 0 100 5 10
    240605174237_7kf3 TCTATTATTTTAGCTGGGGATACGTTTGGATG(+CAGTGT)GATCCACAACAATGGCCCATTCTGCCACAAA NGG 10 8 17 8 24 0 100 5 10
    240605174234_lwkd AAAATGTCCTTGATAATAAATGGTATGGCCCG(+CAGTGT)GATCCTATTTTGATAAGATCCAGGGGAGCTA NGG 10 8 17 8 24 0 100 5 10
    240605174233_w5i0 ACAAATAGCTCCCCTGGATCTTATCAAAATAG(+CAGTGT)GATCCGGGCCATACCATTTATTATCAAGGAC NGG 10 8 17 8 24 0 100 5 10
    240605174231_3r2b ATAGGCCTGTAACTGATCCACTAGGGCAAGGG(+CAGTGT)GTACCTCACAGACAAAAATCACATCCCTAAC NGG 10 8 17 8 24 0 100 5 10