Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605174318_c96n CCTACCTTAAAGTACAGAAATTGTGTGTGATG(+CAGTGT)GTACCTACTTGTAACCCCAGAAACTGGGAGG NGG 10 8 17 8 24 0 100 5 10
    240605174316_00cg CAGACTTGGAGTCTCAGGAGTGTCCAACCAGG(+CAGTGT)GTACCAAGTCCCAGGCCTGTGACCTGGGTTG NGG 10 8 17 8 24 0 100 5 10
    240605174315_d1le GGATCCAACCCAGGTCACAGGCCTGGGACTTG(+CAGTGT)GTACCCTGGTTGGACACTCCTGAGACTCCAA NGG 10 8 17 8 24 0 100 5 10
    240605174314_z302 ACTTGGGGTAAGAAGGGCATCGGTCTGTGAAG(+CAGTGT)GTACCCCTTCTCTCTCAAAAATGGGGATAAG NGG 10 8 17 8 24 0 100 5 10
    240605174312_ljg5 TCAAGCTTATCCCCATTTTTGAGAGAGAAGGG(+CAGTGT)GTACCTTCACAGACCGATGCCCTTCTTACCC NGG 10 8 17 8 24 0 100 5 10
    240605174311_uk9p TCACTGACAGGAGAGCAGCCAGGGGAGGCTAG(+CAGTGT)GTACCTGGGGCTGCTGCGGCTGCCAGAAACC NGG 10 8 17 8 24 0 100 5 10
    240605174307_69xn GAGATGGTTTCTGGCAGCCGCAGCAGCCCCAG(+CAGTGT)GTACCTAGCCTCCCCTGGCTGCTCTCCTGTC NGG 10 8 17 8 24 0 100 5 10
    240605174306_dizn GGTACCAAGTCCCAGGCCTGTGACCTGGGTTG(+CAGTGT)GATCCATGCTATGGGCAGAAGGGGGCTTTGG NGG 10 8 17 8 24 0 100 5 10
    240605174305_54gl CTTTCCCAAAGCCCCCTTCTGCCCATAGCATG(+CAGTGT)GATCCAACCCAGGTCACAGGCCTGGGACTTG NGG 10 8 17 8 24 0 100 5 10
    240605174304_ja9b TTTTGGTTGTTTTGTTTAAAAAGACATTTGAG(+CAGTGT)GATCCAGAGAGATGGGTCAGTGGGTAAAGTC NGG 10 8 17 8 24 0 100 5 10