Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605174403_putw AAAGAGGCGACTCTGGCTTAAGCTAACTGGAG(+CAGTGT)GTACCTTTCAGGGTCCTCTCTCCATTATCAT NGG 10 8 17 8 24 0 100 5 10
    240605174402_nsxe GGCAGCTCAGCCCTTTGCCTTTTCAGGCTATG(+CAGTGT)GATCCCATGTAGCCTGGGAGTTGGCCTCCTC NGG 10 8 17 8 24 0 100 5 10
    240605174400_qo9p TGACTGAGGAGGCCAACTCCCAGGCTACATGG(+CAGTGT)GATCCATAGCCTGAAAAGGCAAAGGGCTGAG NGG 10 8 17 8 24 0 100 5 10
    240605174359_jge2 TGTCCTCTGCCCTATCCCTTATCTCAGGAAGG(+CAGTGT)GTACCTGTGCAAGATGGGAACTGTCCAAGAC NGG 10 8 17 8 24 0 100 5 10
    240605174358_3wnw TCAGGGTCTTGGACAGTTCCCATCTTGCACAG(+CAGTGT)GTACCCTTCCTGAGATAAGGGATAGGGCAGA NGG 10 8 17 8 24 0 100 5 10
    240605174356_bz6i GGTCAGTATCAAGGAGACATGGGTGGCTACAG(+CAGTGT)GATCCCCAAGCTCAGCCCTGGCCCAAGTGAT NGG 10 8 17 8 24 0 100 5 10
    240605174354_i5yd CTGGGATCACTTGGGCCAGGGCTGAGCTTGGG(+CAGTGT)GATCCTGTAGCCACCCATGTCTCCTTGATAC NGG 10 8 17 8 24 0 100 5 10
    240605174353_i7si CTAGGCCAGTGCTACTGTGGACACTGGCTGTG(+CAGTGT)GATCCTGGGCTGCTGCCACTCTGCAGAAGCA NGG 10 8 17 8 24 0 100 5 10
    240605174351_nxbg GTGTATGCTTCTGCAGAGTGGCAGCAGCCCAG(+CAGTGT)GATCCACAGCCAGTGTCCACAGTAGCACTGG NGG 10 8 17 8 24 0 100 5 10
    240605174349_djbu CTTTTGGCCTATTAACATCTCAAATGTGAAGG(+CAGTGT)GTACCATACAGTTCTTAAAAGCCATTCTCAT NGG 10 8 17 8 24 0 100 5 10