Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605165022_lzri GAAAGGAACTCATGCTACAAGATAGAGAGTAG(+CAGTGT)GTACCCAGGAGTGGAGATGGAAGGAGTCTGA NGG 10 8 17 8 24 0 100 5 10
    240605165021_o3as GAGTCAGATTAGTGGTGCTAGGAACTGAGCAG(+CAGTGT)GATCCCCAAGTTTATTTTCTAGAACCTTTAA NGG 10 8 17 8 24 0 100 5 10
    240605165019_2oqy ATTCTTTAAAGGTTCTAGAAAATAAACTTGGG(+CAGTGT)GATCCTGCTCAGTTCCTAGCACCACTAATCT NGG 10 8 17 8 24 0 100 5 10
    240605165017_mqao TATCTCATCTACTCCTGATGTGGGAATCCATG(+CAGTGT)GATCCCTGAGCTTTAATTTGTTCTTGCTTGG NGG 10 8 17 8 24 0 100 5 10
    240605165015_84vu TAAATCCAAGCAAGAACAAATTAAAGCTCAGG(+CAGTGT)GATCCATGGATTCCCACATCAGGAGTAGATG NGG 10 8 17 8 24 0 100 5 10
    240605165014_yldt CCAAAAATAAAAATAAAAAGCTAAACACTATG(+CAGTGT)GATCCTTAGCTAGAAAGCACAGAGATGTGGA NGG 10 8 17 8 24 0 100 5 10
    240605165012_dt1s TCACATCCACATCTCTGTGCTTTCTAGCTAAG(+CAGTGT)GATCCATAGTGTTTAGCTTTTTATTTTTATT NGG 10 8 17 8 24 0 100 5 10
    240605165011_fyqo CAACAGGAAGATCAACAGGGTCAACTAACCTG(+CAGTGT)GATCCTTGGGGCTCTCAGAGACTGAACCACC NGG 10 8 17 8 24 0 100 5 10
    240605165008_te6p CGGTTGGTGGTTCAGTCTCTGAGAGCCCCAAG(+CAGTGT)GATCCAGGTTAGTTGACCCTGTTGATCTTCC NGG 10 8 17 8 24 0 100 5 10
    240605165005_npud TGGGTGGCTTACCTATAATTATAGCTCCAGGG(+CAGTGT)GATCCAATCCCCTCTTCTGGCCTCATCAGGC NGG 10 8 17 8 24 0 100 5 10