Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605164949_0eoc AAAACTAAAAGTATATATTGAGTTGGTCTGAG(+CAGTGT)GATCCTGACTTCTGAGTCTACTGCTAAAAAG NGG 10 8 17 8 24 0 100 5 10
    240605164947_v181 TTGGCTGGTACTTCCTTCCTTCCAGGAGATGG(+CAGTGT)GATCCCTCTAGATACAGGGCCTAGAGACCAA NGG 10 8 17 8 24 0 100 5 10
    240605164945_2uod AGGTTTTGGTCTCTAGGCCCTGTATCTAGAGG(+CAGTGT)GATCCCATCTCCTGGAAGGAAGGAAGTACCA NGG 10 8 17 8 24 0 100 5 10
    240605164943_1fhb ATTTTGCTTCATGTATCCAGGTCCTCTGCAGG(+CAGTGT)GTACCAAGGAATGTTCTTCAGGATAGCGGAA NGG 10 8 17 8 24 0 100 5 10
    240605164941_zdmp AATTGTTCCGCTATCCTGAAGAACATTCCTTG(+CAGTGT)GTACCCTGCAGAGGACCTGGATACATGAAGC NGG 10 8 17 8 24 0 100 5 10
    240605164940_ou9o GTCAAAGTATGTTTAACATATGATTTCTTCAG(+CAGTGT)GATCCCTCCCCTTTCTGAATTCCAAATGGAG NGG 10 8 17 8 24 0 100 5 10
    240605164938_ik4a AGTTCCTCCATTTGGAATTCAGAAAGGGGAGG(+CAGTGT)GATCCTGAAGAAATCATATGTTAAACATACT NGG 10 8 17 8 24 0 100 5 10
    240605164936_cimp TTCTGCCACTCCCTCATGCATGTTTGAGGGTG(+CAGTGT)GATCCCATCCAATGACCAGTAAGAGATTAAA NGG 10 8 17 8 24 0 100 5 10
    240605164935_o0dx AAGCTTTTAATCTCTTACTGGTCATTGGATGG(+CAGTGT)GATCCACCCTCAAACATGCATGAGGGAGTGG NGG 10 8 17 8 24 0 100 5 10
    240605164934_dcrr CCACCCCAGCCAAGAGCTGAGGCGCATGAGAG(+CAGTGT)GATCCTGAGCATCCCCAGAAGGGTGTGTCTG NGG 10 8 17 8 24 0 100 5 10