Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605164038_elm1 GAACTACCTAAATTCCAGTACCAGGGGAGGTG(+CAGTGT)GTACCCTTTTCTGACCTCTGGCACCAGGCAC NGG 10 8 17 8 24 0 100 5 10
    240605164037_tvkc TGGAAAAGCTTGGCAGGCCCCGGGAGGACTCG(+CAGTGT)GATCCATCCCTTTATCTCATCCCTACGCTCC NGG 10 8 17 8 24 0 100 5 10
    240605164035_r6ot ATACTGGAGCGTAGGGATGAGATAAAGGGATG(+CAGTGT)GATCCGAGTCCTCCCGGGGCCTGCCAAGCTT NGG 10 8 17 8 24 0 100 5 10
    240605164034_8z91 ATCTGTCCCATTTCAGAGAGGGCCCTTACTAG(+CAGTGT)GTACCTTTGGTGTGGGGGGCGCTTGGGGAAC NGG 10 8 17 8 24 0 100 5 10
    240605164032_48jl AGCTTGTTCCCCAAGCGCCCCCCACACCAAAG(+CAGTGT)GTACCTAGTAAGGGCCCTCTCTGAAATGGGA NGG 10 8 17 8 24 0 100 5 10
    240605164031_gvrq GGATGTAAACACACTCTGTAAGCTGAAATTCG(+CAGTGT)GTACCCAAATGCATAAGTGGACTTATGACCA NGG 10 8 17 8 24 0 100 5 10
    240605164029_5vjc TGACATGGTCATAAGTCCACTTATGCATTTGG(+CAGTGT)GTACCGAATTTCAGCTTACAGAGTGTGTTTA NGG 10 8 17 8 24 0 100 5 10
    240605164028_tqcm TGTCTTAGTGCAGCAGAAGATAGTTCCAGCAG(+CAGTGT)GTACCGGCTGGGCTTGGTGGCTTTCCAAGCG NGG 10 8 17 8 24 0 100 5 10
    240605164026_b3fb ACGTGCGCTTGGAAAGCCACCAAGCCCAGCCG(+CAGTGT)GTACCTGCTGGAACTATCTTCTGCTGCACTA NGG 10 8 17 8 24 0 100 5 10
    240605164025_6oyh CCATGAATATTAGTGTCCAACAGGTGGAGTCG(+CAGTGT)GTACCCACATTCCTACCCCGCCCCAGACCTG NGG 10 8 17 8 24 0 100 5 10