Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605164127_5mh9 ATTTCCAAGTTGTTAGAACAAGACGGTCAATG(+CAGTGT)GATCCTATCCAGGGAGAGCTGTGACTAATAT NGG 10 8 17 8 24 0 100 5 10
    240605164125_gvga TGAATGTATGTTTTTCCTGTGTGTATCTGTGG(+CAGTGT)GTACCTCATGTGTATGCAGTACCCTTGGAAA NGG 10 8 17 8 24 0 100 5 10
    240605164124_rtxr GCTGGTTTCCAAGGGTACTGCATACACATGAG(+CAGTGT)GTACCCACAGATACACACAGGAAAAACATAC NGG 10 8 17 8 24 0 100 5 10
    240605164122_tl6w TAGTGTTCTAAGGCAACAGCTTCTCCCCACAG(+CAGTGT)GATCCTTCTCCCTCGGCCTTGCTTGGCTACT NGG 10 8 17 8 24 0 100 5 10
    240605164121_qj1f ACACAAGTAGCCAAGCAAGGCCGAGGGAGAAG(+CAGTGT)GATCCTGTGGGGAGAAGCTGTTGCCTTAGAA NGG 10 8 17 8 24 0 100 5 10
    240605164119_9ght GAAGTCAGAGCCCACTTGTCTCCTGGGTACAG(+CAGTGT)GTACCTCATAATGGTCCTGAGATCCAAGGAG NGG 10 8 17 8 24 0 100 5 10
    240605164118_58b1 TGCTGCTCCTTGGATCTCAGGACCATTATGAG(+CAGTGT)GTACCTGTACCCAGGAGACAAGTGGGCTCTG NGG 10 8 17 8 24 0 100 5 10
    240605164116_1z2y CACAGTGAGACCTTGTCTCAAAAAGAGAGGGG(+CAGTGT)GTACCACTAACACCCACCTGGAGGTTCTGAA NGG 10 8 17 8 24 0 100 5 10
    240605164114_vfi1 TTTACTTCAGAACCTCCAGGTGGGTGTTAGTG(+CAGTGT)GTACCCCCTCTCTTTTTGAGACAAGGTCTCA NGG 10 8 17 8 24 0 100 5 10
    240605164113_m5hm TAATCCCAGCGTTGGGGAAACAGAGCTAGAGG(+CAGTGT)GATCCCCATGAGTCCAAGGCTACCCTGGTTT NGG 10 8 17 8 24 0 100 5 10