Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605164219_ay8i AGGAATTCTAGGACAAGGAGAGAAGGTCAGAG(+CAGTGT)GTACCCTCTCTTTTTTGGGAAGATAGCCTGT NGG 10 8 17 8 24 0 100 5 10
    240605164218_0iyy CCAGCTATCCTGCTTCCCAGGGCCACCAACTG(+CAGTGT)GATCCAAGGACCAGGGTGTGTGCAGGCTCCA NGG 10 8 17 8 24 0 100 5 10
    240605164216_jpgv TTTGCTGGAGCCTGCACACACCCTGGTCCTTG(+CAGTGT)GATCCAGTTGGTGGCCCTGGGAAGCAGGATA NGG 10 8 17 8 24 0 100 5 10
    240605164215_u5c7 GCTCATAGAACACGGCGCATGATGGGTAAGAG(+CAGTGT)GATCCAGACTACCCAGTGTGTCCCAGGTATT NGG 10 8 17 8 24 0 100 5 10
    240605164213_mp5q CTGGGAATACCTGGGACACACTGGGTAGTCTG(+CAGTGT)GATCCTCTTACCCATCATGCGCCGTGTTCTA NGG 10 8 17 8 24 0 100 5 10
    240605164212_cey2 TTAGTGTCCTTTAGTCTTCGCACTTGTCTAAG(+CAGTGT)GATCCACTTTTAGGGCTTGCCCACATGTGAT NGG 10 8 17 8 24 0 100 5 10
    240605164210_94lu ATAGAATCACATGTGGGCAAGCCCTAAAAGTG(+CAGTGT)GATCCTTAGACAAGTGCGAAGACTAAAGGAC NGG 10 8 17 8 24 0 100 5 10
    240605164209_dphv GCTGTCTTCAGACCCATTAGAAGAGGGCATCG(+CAGTGT)GATCCCATTATAGATGGTTATAAGCCACCAT NGG 10 8 17 8 24 0 100 5 10
    240605164207_235n ACCACATGGTGGCTTATAACCATCTATAATGG(+CAGTGT)GATCCGATGCCCTCTTCTAATGGGTCTGAAG NGG 10 8 17 8 24 0 100 5 10
    240605164205_mtlq GGTCAGGAGGACCCAGCGCCTACTGCACCCAG(+CAGTGT)GATCCATTAGCACTGTAACAACGAACACCTG NGG 10 8 17 8 24 0 100 5 10