Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605164325_d2ts GTTTGCCAGGAGCCGTTTAGCAGTGTTGCTGG(+CAGTGT)GTACCCAGGGCAGCTAGGATGCTTCAGAGTC NGG 10 8 17 8 24 0 100 5 10
    240605164324_ss79 AGAGGCACTGCCTCTACACTCATAAATTTTTG(+CAGTGT)GTACCAAACTTCTTAAAAACCAGATGGTTAA NGG 10 8 17 8 24 0 100 5 10
    240605164322_kc4x TTTTTTTAACCATCTGGTTTTTAAGAAGTTTG(+CAGTGT)GTACCAAAAATTTATGAGTGTAGAGGCAGTG NGG 10 8 17 8 24 0 100 5 10
    240605164321_pl49 TTTAAAATATAGGTTACCCTGCTACCCTGCTG(+CAGTGT)GATCCTGAGCCTGGAGGCCTGCAGATCCATC NGG 10 8 17 8 24 0 100 5 10
    240605164320_3k32 GCCCGGATGGATCTGCAGGCCTCCAGGCTCAG(+CAGTGT)GATCCAGCAGGGTAGCAGGGTAACCTATATT NGG 10 8 17 8 24 0 100 5 10
    240605164317_bxyg GGCAACTGGGGGGAGGTGGGGTCAGGAACCCG(+CAGTGT)GATCCACCAAGAGGACAGTTCCCAAAGAGGA NGG 10 8 17 8 24 0 100 5 10
    240605164316_57gy CTCCCTCCTCTTTGGGAACTGTCCTCTTGGTG(+CAGTGT)GATCCGGGTTCCTGACCCCACCTCCCCCCAG NGG 10 8 17 8 24 0 100 5 10
    240605164314_5zwk GTGGAGGTGTCTTTAACAAACACTTTACTTTG(+CAGTGT)GTACCAGCACTCAGGTGACACGGCTCAGAGG NGG 10 8 17 8 24 0 100 5 10
    240605164313_h350 AAGGGCCTCTGAGCCGTGTCACCTGAGTGCTG(+CAGTGT)GTACCAAAGTAAAGTGTTTGTTAAAGACACC NGG 10 8 17 8 24 0 100 5 10
    240605164312_plr7 TCACCATTCCTAACAGAAGCACTGCACTGTTG(+CAGTGT)GTACCGAGAAGGGTGGAAGACAGGAGGGGGC NGG 10 8 17 8 24 0 100 5 10