Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605164343_1okz CCTTTTATGCTTTCAAAACCAGTACCATCTGG(+CAGTGT)GTACCTCTTACACGTTACCAAGTCCAGCCAC NGG 10 8 17 8 24 0 100 5 10
    240605164342_7ag4 GCCCTTGGAGGAGCCCAAAGGATCGTGAGTTG(+CAGTGT)GATCCCATACTTTGGATAGTTGGAGTTTAAT NGG 10 8 17 8 24 0 100 5 10
    240605164340_j7sw CCAAAATTAAACTCCAACTATCCAAAGTATGG(+CAGTGT)GATCCAACTCACGATCCTTTGGGCTCCTCCA NGG 10 8 17 8 24 0 100 5 10
    240605164338_76rq TTTTTGGGCTTATTTTGAAGCCACTCCCCAAG(+CAGTGT)GATCCACATTTATTCATGAAAGCAGATATCC NGG 10 8 17 8 24 0 100 5 10
    240605164337_5myk GGTCTGGATATCTGCTTTCATGAATAAATGTG(+CAGTGT)GATCCTTGGGGAGTGGCTTCAAAATAAGCCC NGG 10 8 17 8 24 0 100 5 10
    240605164335_sotu AAATTCAATTTCATAGACTACTGCCCAGCTAG(+CAGTGT)GATCCAGGTCTCAAGCACACAGATGCCTCGC NGG 10 8 17 8 24 0 100 5 10
    240605164333_becy AGTTAGCGAGGCATCTGTGTGCTTGAGACCTG(+CAGTGT)GATCCTAGCTGGGCAGTAGTCTATGAAATTG NGG 10 8 17 8 24 0 100 5 10
    240605164331_4d6g GCCCAAGTTTCTCTATCAGCCACTCCCACCAG(+CAGTGT)GATCCAGCCCCTCAGAGGCACTCAGCTCCTC NGG 10 8 17 8 24 0 100 5 10
    240605164330_dsg6 GGACAGAGGAGCTGAGTGCCTCTGAGGGGCTG(+CAGTGT)GATCCTGGTGGGAGTGGCTGATAGAGAAACT NGG 10 8 17 8 24 0 100 5 10
    240605164327_b8zx GAGAGGACTCTGAAGCATCCTAGCTGCCCTGG(+CAGTGT)GTACCCAGCAACACTGCTAAACGGCTCCTGG NGG 10 8 17 8 24 0 100 5 10