Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605164311_hy6q CCCCCGCCCCCTCCTGTCTTCCACCCTTCTCG(+CAGTGT)GTACCAACAGTGCAGTGCTTCTGTTAGGAAT NGG 10 8 17 8 24 0 100 5 10
    240605164308_lfda ACGGCTCTTTAGGACAACACCACAAACCCAGG(+CAGTGT)GATCCCCAGTGGGGAGGAGGGAACAGATCTT NGG 10 8 17 8 24 0 100 5 10
    240605164306_9gsx CATAAAAGATCTGTTCCCTCCTCCCCACTGGG(+CAGTGT)GATCCCTGGGTTTGTGGTGTTGTCCTAAAGA NGG 10 8 17 8 24 0 100 5 10
    240605164304_lruq ACCAGGACAATGTCACCTCAGGATGGGACTGG(+CAGTGT)GATCCCTGCAAGATGGTTCTACATGGAACCC NGG 10 8 17 8 24 0 100 5 10
    240605164302_mcjk GAGCCGGGTTCCATGTAGAACCATCTTGCAGG(+CAGTGT)GATCCCAGTCCCATCCTGAGGTGACATTGTC NGG 10 8 17 8 24 0 100 5 10
    240605164301_j786 TTTCCAGTCGTCTGAAGCCCGTGCGGGATTTG(+CAGTGT)GATCCTCAGCCACTGCTACGGAGCCTGTGCC NGG 10 8 17 8 24 0 100 5 10
    240605164300_c67o GACAAGGCACAGGCTCCGTAGCAGTGGCTGAG(+CAGTGT)GATCCAAATCCCGCACGGGCTTCAGACGACT NGG 10 8 17 8 24 0 100 5 10
    240605164258_36lg TTAATGTGTTTCTTTGCATAGATGTTACTTAG(+CAGTGT)GTACCGGAATGGGAGAGCAGCGGGGGTGATG NGG 10 8 17 8 24 0 100 5 10
    240605164257_6024 TGGATCATCACCCCCGCTGCTCTCCCATTCCG(+CAGTGT)GTACCTAAGTAACATCTATGCAAAGAAACAC NGG 10 8 17 8 24 0 100 5 10
    240605164255_k94a TGTGATACCAGCATGCCCTGATACGCCGTTAG(+CAGTGT)GTACCACAGGTCTACTACTGGGAGAGCTATG NGG 10 8 17 8 24 0 100 5 10